A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin
Herein, we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleav...
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2020-10-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177920310510 |
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doaj-9efea5b60175443481393ee4f57339072021-04-02T17:00:17ZengElsevierJournal of Pharmaceutical Analysis2095-17792020-10-01105482489A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsinShanshan Hou0Tingting Feng1Na Zhao2Jiaxin Zhang3Huibin Wang4Ning Liang5Longshan Zhao6School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, ChinaInstitute of Pharmaceutical and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong, Shanxi Province, 030619, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, ChinaSchool of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, China; Corresponding author.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, 110016, China; Corresponding author.Herein, we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleave the peptide, resulting in the release of the dye moiety and a substantial increase in fluorescence intensity, which was dose- and time-dependent, and trypsin could be quantified accordingly. Correspondingly, the biosensor has led to the development of a convenient and efficient fluorescent method to measure trypsin activity, with a detection limit of 0.7 μg/mL. The method allows rapid determination of trypsin activity in the normal and acute pancreatitis range, suitable for point-of-care testing. Furthermore, the applicability of the method has been demonstrated by detecting trypsin in spiked urine samples.http://www.sciencedirect.com/science/article/pii/S2095177920310510Carbon nanoparticlesFluorescence quenchingFörster resonance energy transfer (FRET)Fluorescein-labelled peptideTrypsin assay |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Shanshan Hou Tingting Feng Na Zhao Jiaxin Zhang Huibin Wang Ning Liang Longshan Zhao |
| spellingShingle |
Shanshan Hou Tingting Feng Na Zhao Jiaxin Zhang Huibin Wang Ning Liang Longshan Zhao A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin Journal of Pharmaceutical Analysis Carbon nanoparticles Fluorescence quenching Förster resonance energy transfer (FRET) Fluorescein-labelled peptide Trypsin assay |
| author_facet |
Shanshan Hou Tingting Feng Na Zhao Jiaxin Zhang Huibin Wang Ning Liang Longshan Zhao |
| author_sort |
Shanshan Hou |
| title |
A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin |
| title_short |
A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin |
| title_full |
A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin |
| title_fullStr |
A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin |
| title_full_unstemmed |
A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin |
| title_sort |
carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin |
| publisher |
Elsevier |
| series |
Journal of Pharmaceutical Analysis |
| issn |
2095-1779 |
| publishDate |
2020-10-01 |
| description |
Herein, we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleave the peptide, resulting in the release of the dye moiety and a substantial increase in fluorescence intensity, which was dose- and time-dependent, and trypsin could be quantified accordingly. Correspondingly, the biosensor has led to the development of a convenient and efficient fluorescent method to measure trypsin activity, with a detection limit of 0.7 μg/mL. The method allows rapid determination of trypsin activity in the normal and acute pancreatitis range, suitable for point-of-care testing. Furthermore, the applicability of the method has been demonstrated by detecting trypsin in spiked urine samples. |
| topic |
Carbon nanoparticles Fluorescence quenching Förster resonance energy transfer (FRET) Fluorescein-labelled peptide Trypsin assay |
| url |
http://www.sciencedirect.com/science/article/pii/S2095177920310510 |
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