Genomic Characterization and Expression Analysis of the SnRK family genes in <i>Dendrobium officinale</i> Kimura et Migo (Orchidaceae)

• Main Authors: Yue Wang, Aizhong Liu
• Format: Article
• Language: English
• Published: MDPI AG 2021-03-01
• Series: Plants
• Subjects: sucrose non-fermenting1-related protein kinases (SnRKs); <i>Dendrobium officinale</i>; expression patterns; stress
• Online Access: https://www.mdpi.com/2223-7747/10/3/479

Sucrose non-fermenting1-related protein kinases (SnRKs) are a type of Ser/Thr protein kinases, and they play an important role in plant life, especially in metabolism and responses to environmental stresses. However, there is limited information on <i>SnRK</i> genes in <i>Dendrobium officinale</i>. In the present research, a total of 36 <i>DoSnRK</i> genes were identified based on genomic data. These DoSnRKs could be grouped into three subfamilies, including 1 member of DoSnRK1, 7 of DoSnRK2, and 28 of DoSnRK3. The gene structure analysis of <i>DoSnRK</i> genes showed that 17 members had no introns, while 16 members contained six or more introns. The conserved domains and motifs were found in the same subfamily. The various cis-elements present in the promoter regions showed that <i>DoSnRK</i> genes could respond to stresses and hormones. Furthermore, the expression patterns of <i>DoSnRK</i> genes in eight tissues were investigated according to RNA sequencing data, indicating that multiple <i>DoSnRK</i> genes were ubiquitously expressed in these tissues. The transcript levels of <i>DoSnRK</i> genes after drought, MeJA, and ABA treatments were analyzed by quantitative real-time PCR and showed that most <i>DoSnRK</i> genes could respond to these stresses. Therefore, genomic characterization and expression analyses provide valuable information on <i>DoSnRK</i> genes for further understanding the functions of SnRKs in plants.