Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder
Local synthesis of antibodies and presence of oligoclonal bands in the cerebrospinal fluid (CSF) are hallmarks of multiple sclerosis (MS). We investigated the frequency of antibodies against mycobacterial and relevant human epitopes in the CSF of patients with MS or neuromyelitis optica spectrum dis...
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/7/12/522 |
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doaj-9ea77f73f8b144b6b5e1511a102534972020-11-24T21:23:02ZengMDPI AGJournal of Clinical Medicine2077-03832018-12-0171252210.3390/jcm7120522jcm7120522Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum DisorderKazumasa Yokoyama0Davide Cossu1Yasunobu Hoshino2Yuji Tomizawa3Eiichi Momotani4Nobutaka Hattori5Department of Neurology, Juntendo University School of Medicine, Tokyo 113-8421, JapanDepartment of Neurology, Juntendo University School of Medicine, Tokyo 113-8421, JapanDepartment of Neurology, Juntendo University School of Medicine, Tokyo 113-8421, JapanDepartment of Neurology, Juntendo University School of Medicine, Tokyo 113-8421, JapanDepartment of Human-care, Tohto College of Health Sciences, Saitama 366-0052, JapanDepartment of Neurology, Juntendo University School of Medicine, Tokyo 113-8421, JapanLocal synthesis of antibodies and presence of oligoclonal bands in the cerebrospinal fluid (CSF) are hallmarks of multiple sclerosis (MS). We investigated the frequency of antibodies against mycobacterial and relevant human epitopes in the CSF of patients with MS or neuromyelitis optica spectrum disorder (NMOSD) and whether these antibodies differed from those present in the serum. Matched serum and CSF samples from 46 patients with MS, 42 patients with NMOSD, and 29 age-matched and sex-matched control subjects were screened retrospectively for the presence of antibodies against <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) pentapeptide (MAP_5p), MAP_2694<sub>295⁻303</sub>, and myelin basic protein (MBP)<sub>85⁻98</sub> peptides by using indirect ELISA. Serum levels of anti-MAP_5p and anti-MAP_2694<sub>295⁻303</sub> antibodies were highly prevalent in patients with MS when compared to patients with NMOSD and controls. Several patients with MS had detectable anti-MAP_5p and anti-MAP_2694<sub>295⁻303</sub> antibodies in the CSF. Furthermore, a group of patients with MS showed intrathecally restricted production of antibodies against these peptides. Women appeared to mount a stronger humoral response to mycobacterial peptides than men. No significant difference in the frequency of anti-MBP<sub>85⁻98</sub> antibodies was found between patients with MS and those with NMOSD. These data highlight the zoonotic potential of MAP, which suggests its involvement in MS etiopathogenesis.https://www.mdpi.com/2077-0383/7/12/522MycobacteriumJapanhumoral responsecerebrospinal fluidmultiple sclerosisNMOSDMBP |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Kazumasa Yokoyama Davide Cossu Yasunobu Hoshino Yuji Tomizawa Eiichi Momotani Nobutaka Hattori |
| spellingShingle |
Kazumasa Yokoyama Davide Cossu Yasunobu Hoshino Yuji Tomizawa Eiichi Momotani Nobutaka Hattori Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder Journal of Clinical Medicine Mycobacterium Japan humoral response cerebrospinal fluid multiple sclerosis NMOSD MBP |
| author_facet |
Kazumasa Yokoyama Davide Cossu Yasunobu Hoshino Yuji Tomizawa Eiichi Momotani Nobutaka Hattori |
| author_sort |
Kazumasa Yokoyama |
| title |
Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder |
| title_short |
Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder |
| title_full |
Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder |
| title_fullStr |
Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder |
| title_full_unstemmed |
Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder |
| title_sort |
anti-mycobacterial antibodies in paired cerebrospinal fluid and serum samples from japanese patients with multiple sclerosis or neuromyelitis optica spectrum disorder |
| publisher |
MDPI AG |
| series |
Journal of Clinical Medicine |
| issn |
2077-0383 |
| publishDate |
2018-12-01 |
| description |
Local synthesis of antibodies and presence of oligoclonal bands in the cerebrospinal fluid (CSF) are hallmarks of multiple sclerosis (MS). We investigated the frequency of antibodies against mycobacterial and relevant human epitopes in the CSF of patients with MS or neuromyelitis optica spectrum disorder (NMOSD) and whether these antibodies differed from those present in the serum. Matched serum and CSF samples from 46 patients with MS, 42 patients with NMOSD, and 29 age-matched and sex-matched control subjects were screened retrospectively for the presence of antibodies against <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) pentapeptide (MAP_5p), MAP_2694<sub>295⁻303</sub>, and myelin basic protein (MBP)<sub>85⁻98</sub> peptides by using indirect ELISA. Serum levels of anti-MAP_5p and anti-MAP_2694<sub>295⁻303</sub> antibodies were highly prevalent in patients with MS when compared to patients with NMOSD and controls. Several patients with MS had detectable anti-MAP_5p and anti-MAP_2694<sub>295⁻303</sub> antibodies in the CSF. Furthermore, a group of patients with MS showed intrathecally restricted production of antibodies against these peptides. Women appeared to mount a stronger humoral response to mycobacterial peptides than men. No significant difference in the frequency of anti-MBP<sub>85⁻98</sub> antibodies was found between patients with MS and those with NMOSD. These data highlight the zoonotic potential of MAP, which suggests its involvement in MS etiopathogenesis. |
| topic |
Mycobacterium Japan humoral response cerebrospinal fluid multiple sclerosis NMOSD MBP |
| url |
https://www.mdpi.com/2077-0383/7/12/522 |
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