Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]

Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic modification of RNA. These methods have differen...

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Main Authors: Lilith V.J.C. Mannack, Sebastian Eising, Andrea Rentmeister
Format: Article
Language:English
Published: F1000 Research Ltd 2016-04-01
Series:F1000Research
Subjects:
Online Access:http://f1000research.com/articles/5-775/v1
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spelling doaj-9e6be7f54afe48cfa2725af466d6f5472020-11-25T03:20:36ZengF1000 Research LtdF1000Research2046-14022016-04-01510.12688/f1000research.8151.18767Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]Lilith V.J.C. Mannack0Sebastian Eising1Andrea Rentmeister2Cells in Motion, Cluster of Excellence, Münster, GermanyDepartment of Chemistry, Institute of Biochemistry, University of Münster, Münster, GermanyCells in Motion, Cluster of Excellence, Münster, GermanyLabeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic modification of RNA. These methods have different benefits and limitations. This review aims to outline the current state-of-the-art techniques and point out their benefits and limitations.http://f1000research.com/articles/5-775/v1Chemical Biology of the CellControl of Gene ExpressionGenomics
collection DOAJ
language English
format Article
sources DOAJ
author Lilith V.J.C. Mannack
Sebastian Eising
Andrea Rentmeister
spellingShingle Lilith V.J.C. Mannack
Sebastian Eising
Andrea Rentmeister
Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]
F1000Research
Chemical Biology of the Cell
Control of Gene Expression
Genomics
author_facet Lilith V.J.C. Mannack
Sebastian Eising
Andrea Rentmeister
author_sort Lilith V.J.C. Mannack
title Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]
title_short Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]
title_full Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]
title_fullStr Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]
title_full_unstemmed Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]
title_sort current techniques for visualizing rna in cells [version 1; referees: 2 approved]
publisher F1000 Research Ltd
series F1000Research
issn 2046-1402
publishDate 2016-04-01
description Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic modification of RNA. These methods have different benefits and limitations. This review aims to outline the current state-of-the-art techniques and point out their benefits and limitations.
topic Chemical Biology of the Cell
Control of Gene Expression
Genomics
url http://f1000research.com/articles/5-775/v1
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