A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity

A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity

A rapid IgM-detection immunochromatographic test (WITNESS® Lepto, Zoetis) has recently become available to identify acute canine leptospirosis at the point of care. Diagnostic sensitivity and specificity of the test were evaluated by comparison with the microscopic agglutination assay (MAT), using a...

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Main Authors: Angeli Kodjo, Christophe Calleja, Michael Loenser, Dan Lin, Joshua Lizer
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2016/3760191
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spelling doaj-9e3673125fc54a07afe26eff3b653f2c2020-11-24T23:49:55ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/37601913760191A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and SpecificityAngeli Kodjo0Christophe Calleja1Michael Loenser2Dan Lin3Joshua Lizer4Laboratoire des Leptospires, VetAgro Sup, Campus Veterinaire de Lyon, 1 avenue Bourgelat, 69280 Marcy l’Etoile, FranceZoetis Global Diagnostics, 100 Campus Drive, Florham Park, NJ 07932, USAZoetis Global Diagnostics, 100 Campus Drive, Florham Park, NJ 07932, USAZoetis Veterinary Medicine Research & Development, Hoge Wei 10, 1930 Zaventem, BelgiumZoetis Veterinary Medicine Research & Development, 333 Portage Street, Kalamazoo, MI 49007, USAA rapid IgM-detection immunochromatographic test (WITNESS® Lepto, Zoetis) has recently become available to identify acute canine leptospirosis at the point of care. Diagnostic sensitivity and specificity of the test were evaluated by comparison with the microscopic agglutination assay (MAT), using a positive cut-off titer of ≥800. Banked serum samples from dogs exhibiting clinical signs and suspected leptospirosis were selected to form three groups based on MAT titer: (1) positive (n=50); (2) borderline (n=35); and (3) negative (n=50). Using an analysis to weight group sizes to reflect French prevalence, the sensitivity and specificity were 98% and 93.5% (88.2% unweighted), respectively. This test rapidly identifies cases of acute canine leptospirosis with high levels of sensitivity and specificity with no interference from previous vaccination.http://dx.doi.org/10.1155/2016/3760191
collection DOAJ
language English
format Article
sources DOAJ
author Angeli Kodjo
Christophe Calleja
Michael Loenser
Dan Lin
Joshua Lizer
spellingShingle Angeli Kodjo
Christophe Calleja
Michael Loenser
Dan Lin
Joshua Lizer
A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity
BioMed Research International
author_facet Angeli Kodjo
Christophe Calleja
Michael Loenser
Dan Lin
Joshua Lizer
author_sort Angeli Kodjo
title A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity
title_short A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity
title_full A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity
title_fullStr A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity
title_full_unstemmed A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity
title_sort rapid in-clinic test detects acute leptospirosis in dogs with high sensitivity and specificity
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2016-01-01
description A rapid IgM-detection immunochromatographic test (WITNESS® Lepto, Zoetis) has recently become available to identify acute canine leptospirosis at the point of care. Diagnostic sensitivity and specificity of the test were evaluated by comparison with the microscopic agglutination assay (MAT), using a positive cut-off titer of ≥800. Banked serum samples from dogs exhibiting clinical signs and suspected leptospirosis were selected to form three groups based on MAT titer: (1) positive (n=50); (2) borderline (n=35); and (3) negative (n=50). Using an analysis to weight group sizes to reflect French prevalence, the sensitivity and specificity were 98% and 93.5% (88.2% unweighted), respectively. This test rapidly identifies cases of acute canine leptospirosis with high levels of sensitivity and specificity with no interference from previous vaccination.
url http://dx.doi.org/10.1155/2016/3760191
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