In vitro cytotoxicity of chemical preservatives on human fibroblast cells

• Main Authors: Daniel Gonsales Spindola, Andre Hinsberger, Valéria Maria de Souza Antunes, Luis Felipe Gomes Michelin, Claudia Bincoletto, Carlos Rocha Oliveira
• Format: Article
• Language: English
• Published: Universidade de São Paulo 2018-05-01
• Series: Brazilian Journal of Pharmaceutical Sciences
• Subjects: Preservatives; Pharmaceutical/chemistry; Fibroblasts/cytotoxicity; Cell death/drug effects; Cosmetics/additives
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100603&lng=en&tlng=en

ABSTRACT Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MTT assay and propidium iodide staining (IMU>methylparaben>sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells. Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors.