Next generation shotgun sequencing and the challenges of de novo genome assembly

Sequencing the genomes of the many scientifically fascinating plants and animals found in South Africa is fast becoming a viable option as a result of the rapid and sustained drop in the cost of next generation sequencing over the last five years. However, the processing and assembly of the sequence...

Full description

Bibliographic Details
Main Authors:	Stephen Schlebusch, Nicola Illing
Format:	Article
Language:	English
Published:	Academy of Science of South Africa 2012-10-01
Series:	South African Journal of Science
Online Access:	http://www.sajs.co.za/index.php/SAJS/article/view/1256

id	doaj-9e20adfd65d640cdb355d1d09e5a06ed
record_format	Article
spelling	doaj-9e20adfd65d640cdb355d1d09e5a06ed2020-11-24T23:24:07ZengAcademy of Science of South AfricaSouth African Journal of Science0038-23531996-74892012-10-0110811-1218Next generation shotgun sequencing and the challenges of de novo genome assemblyStephen SchlebuschNicola IllingSequencing the genomes of the many scientifically fascinating plants and animals found in South Africa is fast becoming a viable option as a result of the rapid and sustained drop in the cost of next generation sequencing over the last five years. However, the processing and assembly of the sequence data produced is not trivial. There are several factors which need to be taken into consideration when planning a strategy to assemble genome sequence data de novo. This paper reviews the advances and the challenges in two of the most rapidly developing areas of the field: the sequencing technology and the software programs used to assemble de novo the sequence data generated by these technologies into a genome.http://www.sajs.co.za/index.php/SAJS/article/view/1256
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Stephen Schlebusch Nicola Illing
spellingShingle	Stephen Schlebusch Nicola Illing Next generation shotgun sequencing and the challenges of de novo genome assembly South African Journal of Science
author_facet	Stephen Schlebusch Nicola Illing
author_sort	Stephen Schlebusch
title	Next generation shotgun sequencing and the challenges of de novo genome assembly
title_short	Next generation shotgun sequencing and the challenges of de novo genome assembly
title_full	Next generation shotgun sequencing and the challenges of de novo genome assembly
title_fullStr	Next generation shotgun sequencing and the challenges of de novo genome assembly
title_full_unstemmed	Next generation shotgun sequencing and the challenges of de novo genome assembly
title_sort	next generation shotgun sequencing and the challenges of de novo genome assembly
publisher	Academy of Science of South Africa
series	South African Journal of Science
issn	0038-2353 1996-7489
publishDate	2012-10-01
description	Sequencing the genomes of the many scientifically fascinating plants and animals found in South Africa is fast becoming a viable option as a result of the rapid and sustained drop in the cost of next generation sequencing over the last five years. However, the processing and assembly of the sequence data produced is not trivial. There are several factors which need to be taken into consideration when planning a strategy to assemble genome sequence data de novo. This paper reviews the advances and the challenges in two of the most rapidly developing areas of the field: the sequencing technology and the software programs used to assemble de novo the sequence data generated by these technologies into a genome.
url	http://www.sajs.co.za/index.php/SAJS/article/view/1256
work_keys_str_mv	AT stephenschlebusch nextgenerationshotgunsequencingandthechallengesofdenovogenomeassembly AT nicolailling nextgenerationshotgunsequencingandthechallengesofdenovogenomeassembly
_version_	1725561718855172096

Next generation shotgun sequencing and the challenges of de novo genome assembly

Similar Items