DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD

DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD

The present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ng∙mL-1 (3-MT) and 250 ng∙mL-1 (DOPAC). The method accuracy has...

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Main Authors: RALUCA-IOANA TAMPU 1, 2, ADRIANA FINARU, CLAIRE ELFAKIR
Format: Article
Language:English
Published: Alma Mater Publishing House "Vasile Alecsandri" University of Bacau 2020-03-01
Series:Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
Subjects:
hilic
neurotransmitters
sheep brain extract
tandem mass spectrometry
tsk gel amide 80.
Online Access:http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202001&vol=1&aid=5094
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spelling doaj-9e18aa9a6bfe411e9fd25149e73337462020-11-25T02:32:08ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X2020-03-012115969DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHODRALUCA-IOANA TAMPU 1, 20ADRIANA FINARU1 CLAIRE ELFAKIR21. “Vasile Alecsandri” University of Bacau, Calea Marasesti, 157, Bacau-600115, Romania 2. Institute of Organic and Analytical Chemistry CNRS UMR 7311, University of Orléans, Chartres, BP 6759, 45067 Orléans, France“Vasile Alecsandri” University of Bacau, Calea Marasesti, 157, Bacau-600115, RomaniaInstitute of Organic and Analytical Chemistry CNRS UMR 7311, University of Orléans, Chartres, BP 6759, 45067 Orléans, FranceThe present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ng∙mL-1 (3-MT) and 250 ng∙mL-1 (DOPAC). The method accuracy has been evaluated in brain extract by recovery studies with samples spiked at a concentration of 100 ng∙mL-1. For the spiked brain extract, the mean recovery values ranged from 81 % to 101 %, with precision < 8 %.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202001&vol=1&aid=5094hilicneurotransmitterssheep brain extracttandem mass spectrometrytsk gel amide 80.
collection DOAJ
language English
format Article
sources DOAJ
author RALUCA-IOANA TAMPU 1, 2
ADRIANA FINARU
CLAIRE ELFAKIR
spellingShingle RALUCA-IOANA TAMPU 1, 2
ADRIANA FINARU
CLAIRE ELFAKIR
DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
hilic
neurotransmitters
sheep brain extract
tandem mass spectrometry
tsk gel amide 80.
author_facet RALUCA-IOANA TAMPU 1, 2
ADRIANA FINARU
CLAIRE ELFAKIR
author_sort RALUCA-IOANA TAMPU 1, 2
title DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
title_short DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
title_full DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
title_fullStr DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
title_full_unstemmed DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD
title_sort determination of catecholamines and related molecules in brain extract using a hydrophilic interaction liquid chromatography mass spectrometry method
publisher Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
series Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
issn 1582-540X
publishDate 2020-03-01
description The present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ng∙mL-1 (3-MT) and 250 ng∙mL-1 (DOPAC). The method accuracy has been evaluated in brain extract by recovery studies with samples spiked at a concentration of 100 ng∙mL-1. For the spiked brain extract, the mean recovery values ranged from 81 % to 101 %, with precision < 8 %.
topic hilic
neurotransmitters
sheep brain extract
tandem mass spectrometry
tsk gel amide 80.
url http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202001&vol=1&aid=5094
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AT adrianafinaru determinationofcatecholaminesandrelatedmoleculesinbrainextractusingahydrophilicinteractionliquidchromatographymassspectrometrymethod
AT claireelfakir determinationofcatecholaminesandrelatedmoleculesinbrainextractusingahydrophilicinteractionliquidchromatographymassspectrometrymethod
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