Recombinant expression of monovalent and bivalent anti-TNT-antibodies – evaluation of different expression systems

Recombinant expression of monovalent and bivalent anti-TNT-antibodies – evaluation of different expression systems

Monoclonal 11B3 anti-TNT (trinitrotoluene) antibody was expressed as a monovalent and bivalent form using different prokaryotic and eukaryotic expression systems. Recombinant expression in Escherichia coli, mammalian cells and the methylotrophic yeast Pichia pastoris was performed to obtain disulfid...

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Bibliographic Details
Main Authors: BRANISLAV SIMONOVIC, MIROSLAV M. VRVIC, SVETLANA ZLATANOVIC-MILOSEVIC, MLADEN SIMONOVIC
Format: Article
Language:English
Published: Serbian Chemical Society 2008-02-01
Series:Journal of the Serbian Chemical Society
Subjects:
TNT
antibody
recombinant expression
11B3
scFv
Online Access:http://www.shd.org.yu/JSCS/Vol73/No2/JSCS-3696.pdf
Description
Summary:Monoclonal 11B3 anti-TNT (trinitrotoluene) antibody was expressed as a monovalent and bivalent form using different prokaryotic and eukaryotic expression systems. Recombinant expression in Escherichia coli, mammalian cells and the methylotrophic yeast Pichia pastoris was performed to obtain disulfide-linked and glycosylated antibody forms. The generation of antibody and subsequent evaluation of the expression rates were performed using intracellular, excretory and periplasmatic expression techniques. All methods involved striving for native expressed antibody with maintenance of its functionality only.
ISSN:0352-5139

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