Formation of the Secondary Abscission Zone Induced by the Interaction of Methyl Jasmonate and Auxin in <i>Bryophyllum calycinum</i>: Relevance to Auxin Status and Histology

• Main Authors: Agnieszka Marasek-Ciolakowska, Marian Saniewski, Michał Dziurka, Urszula Kowalska, Justyna Góraj-Koniarska, Junichi Ueda, Kensuke Miyamoto
• Format: Article
• Language: English
• Published: MDPI AG 2020-04-01
• Series: International Journal of Molecular Sciences
• Subjects: auxin; abscission; <i>Bryophyllum calycinum</i>; histology; hormonal crosstalk; methyl jasmonate
• Online Access: https://www.mdpi.com/1422-0067/21/8/2784
• ISSN: 1661-6596; 1422-0067

The interaction of methyl jasmonate (JA-Me) and indole-3-acetic acid (IAA) to induce the formation of the secondary abscission zone in the middle of internode segments of <i>Bryophyllum calycinum</i> was investigated in relation to auxin status and histology. When IAA at 0.1% (<i>w</i>/<i>w</i>, in lanolin) was applied to the segments, the formation of the secondary abscission zone at a few mm above the treatment in the apical direction was observed. On the contrary, IAA at 0.5% (<i>w</i>/<i>w</i>, in lanolin) did not induce the formation of the secondary abscission zone. JA-Me at 0.5% (<i>w</i>/<i>w</i>, in lanolin) applied to the middle of internode segments kept in the normal (natural) or inverted positions also induced the formation of the secondary abscission zone below and above parts of the treatment. IAA at 0.5% applied to the cut surface of the upper part of the segments completely prevented the formation of the secondary abscission zone induced by JA-Me. Simultaneous application of IAA 0.5% with JA-Me 0.5% in the middle part of the internode segments induced the formation of the secondary abscission zone at 10 mm to 12 mm above the treatment. Histological analyses indicated that the formation of the secondary abscission zone was characterized by the presence of newly synthesized cell plates that resulted from periclinal cell division within one layer of mother cells in stems. The effects of IAA (0.1%) and JA-Me (0.5%) on the formation of the secondary abscission zone were histologically similar. Comprehensive analyses of plant hormones revealed that the balance of the endogenous levels of IAA in both sides adjacent to the abscission zone was significantly disturbed when the secondary abscission formation was induced by the application of IAA. These results strongly suggest that an auxin gradient is important in the formation of the secondary abscission zone in the internode segments of<i> B. calycinum</i>, and IAA gradient results from polar IAA transport from the application site. IAA is important in the regulation of formation of the secondary abscission zone induced by JA-Me. Further possible mechanisms of the formation of the secondary abscission zone in the internode segments of <i>B. calycinum</i> are also discussed in the interaction of JA-Me and IAA.