Single-molecule detection on a portable 3D-printed microscope

Single-molecule detection on a portable 3D-printed microscope

Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-s...

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Main Authors: James W. P. Brown, Arnaud Bauer, Mark E Polinkovsky, Akshay Bhumkar, Dominic J. B. Hunter, Katharina Gaus, Emma Sierecki, Yann Gambin
Format: Article
Language:English
Published: Nature Publishing Group 2019-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-13617-0
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spelling doaj-9dbd763de888423491044a480dfdd1b92021-05-11T11:45:12ZengNature Publishing GroupNature Communications2041-17232019-12-011011710.1038/s41467-019-13617-0Single-molecule detection on a portable 3D-printed microscopeJames W. P. Brown0Arnaud Bauer1Mark E Polinkovsky2Akshay Bhumkar3Dominic J. B. Hunter4Katharina Gaus5Emma Sierecki6Yann Gambin7EMBL Australia Node in Single Molecule Science, and School of Medical Sciences, University of New South WalesEMBL Australia Node in Single Molecule Science, and School of Medical Sciences, University of New South WalesEMBL Australia Node in Single Molecule Science, and School of Medical Sciences, University of New South WalesEMBL Australia Node in Single Molecule Science, and School of Medical Sciences, University of New South WalesThe Institute for Molecular Bioscience, University of QueenslandEMBL Australia Node in Single Molecule Science, and School of Medical Sciences, University of New South WalesEMBL Australia Node in Single Molecule Science, and School of Medical Sciences, University of New South WalesEMBL Australia Node in Single Molecule Science, and School of Medical Sciences, University of New South WalesSingle-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-synuclein aggregation.https://doi.org/10.1038/s41467-019-13617-0
collection DOAJ
language English
format Article
sources DOAJ
author James W. P. Brown
Arnaud Bauer
Mark E Polinkovsky
Akshay Bhumkar
Dominic J. B. Hunter
Katharina Gaus
Emma Sierecki
Yann Gambin
spellingShingle James W. P. Brown
Arnaud Bauer
Mark E Polinkovsky
Akshay Bhumkar
Dominic J. B. Hunter
Katharina Gaus
Emma Sierecki
Yann Gambin
Single-molecule detection on a portable 3D-printed microscope
Nature Communications
author_facet James W. P. Brown
Arnaud Bauer
Mark E Polinkovsky
Akshay Bhumkar
Dominic J. B. Hunter
Katharina Gaus
Emma Sierecki
Yann Gambin
author_sort James W. P. Brown
title Single-molecule detection on a portable 3D-printed microscope
title_short Single-molecule detection on a portable 3D-printed microscope
title_full Single-molecule detection on a portable 3D-printed microscope
title_fullStr Single-molecule detection on a portable 3D-printed microscope
title_full_unstemmed Single-molecule detection on a portable 3D-printed microscope
title_sort single-molecule detection on a portable 3d-printed microscope
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-12-01
description Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-synuclein aggregation.
url https://doi.org/10.1038/s41467-019-13617-0
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