Single-molecule detection on a portable 3D-printed microscope

Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-s...

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Bibliographic Details
Main Authors: James W. P. Brown, Arnaud Bauer, Mark E Polinkovsky, Akshay Bhumkar, Dominic J. B. Hunter, Katharina Gaus, Emma Sierecki, Yann Gambin
Format: Article
Language:English
Published: Nature Publishing Group 2019-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-13617-0