Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR

Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR

The backbone assignment of medium-sized proteins is rarely as straightforward as that of small proteins, and thus often requires creative solutions. Here, we describe the application of a combination of standard 3D heteronuclear methods with CC(CO)NH and a variety of MUltiplicity Selective In-phase...

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Bibliographic Details
Main Authors: Annette K. Brenner, Nils Åge Frøystein
Format: Article
Language:English
Published: MDPI AG 2014-06-01
Series:Molecules
Subjects:
protein NMR spectroscopy
backbone assignment
CC(CO)NH
MUSIC
Online Access:http://www.mdpi.com/1420-3049/19/7/8890

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