Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion method

A study has been performed on the antimicrobial activities of "jambu biji" (Psidium guajava Linn) leaves from several cultivars(red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that...

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Main Authors: Farida Lanawati Darsono, Stephanie Devi Artemisia
Format: Article
Language:English
Published: Indonesian Biological Society 2003-06-01
Series:Journal of Biological Researches
Subjects:
Online Access:http://berkalahayati.org/journal/06f52e91
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spelling doaj-9db00db9d0404dd09eb8b1dd4de665372020-11-24T21:31:54ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2003-06-0191495110.23869/bphjbr.9.1.200310Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion methodFarida Lanawati Darsono0Stephanie Devi Artemisia1Fakultas Farmasi-Universitas Katolik Widya MandalaFakultas Farmasi-Universitas Katolik Widya MandalaA study has been performed on the antimicrobial activities of "jambu biji" (Psidium guajava Linn) leaves from several cultivars(red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of "jambu biji" are frequently used in traditional medicine as a remedy against diarrhea.The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of "jambu biji" for each cultivar were obtained by reflux with ethanol 96%. The concentrations of the extracts applied to the holes were 10%, 20%, and 30% w/v, the extracts were reconstituted with tween 80 and ethanol 96%. Based on the result of the study, it can be concluded that the extract of "jambu biji" from each cultivar with the concentration of 10%, 20%, and 30%w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anova Factorial 3×3 and furthery tested for significancy (α = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923. http://berkalahayati.org/journal/06f52e91DiareStaphylococcus aureusPsidium guajava LinnCultivarHole plate method
collection DOAJ
language English
format Article
sources DOAJ
author Farida Lanawati Darsono
Stephanie Devi Artemisia
spellingShingle Farida Lanawati Darsono
Stephanie Devi Artemisia
Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion method
Journal of Biological Researches
Diare
Staphylococcus aureus
Psidium guajava Linn
Cultivar
Hole plate method
author_facet Farida Lanawati Darsono
Stephanie Devi Artemisia
author_sort Farida Lanawati Darsono
title Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion method
title_short Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion method
title_full Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion method
title_fullStr Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion method
title_full_unstemmed Aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap Staphylococcus aureus ATCC 25923 dengan holeplate diffusion method
title_sort aktivitas antimikroba ekstrak daun jambu biji dari beberapa kultivar terhadap staphylococcus aureus atcc 25923 dengan holeplate diffusion method
publisher Indonesian Biological Society
series Journal of Biological Researches
issn 0852-6834
2337-389X
publishDate 2003-06-01
description A study has been performed on the antimicrobial activities of "jambu biji" (Psidium guajava Linn) leaves from several cultivars(red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of "jambu biji" are frequently used in traditional medicine as a remedy against diarrhea.The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of "jambu biji" for each cultivar were obtained by reflux with ethanol 96%. The concentrations of the extracts applied to the holes were 10%, 20%, and 30% w/v, the extracts were reconstituted with tween 80 and ethanol 96%. Based on the result of the study, it can be concluded that the extract of "jambu biji" from each cultivar with the concentration of 10%, 20%, and 30%w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anova Factorial 3×3 and furthery tested for significancy (α = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.
topic Diare
Staphylococcus aureus
Psidium guajava Linn
Cultivar
Hole plate method
url http://berkalahayati.org/journal/06f52e91
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