Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene

Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene

Transgene free UNIPDi002-A-human induced pluripotent stem cell (hiPSC) line was generated by Sendai Virus Vectors reprogramming from human oral mucosal epithelial stem cells (hOMESCs) of a patient affected by ectrodactyly-ectodermal dysplasia-clefting (EEC)-syndrome, carrying a mutation in exon 8 of...

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Main Authors: Marta Trevisan, Vanessa Barbaro, Silvia Riccetti, Giulia Masi, Luisa Barzon, Patrizia Nespeca, Gualtiero Alvisi, Enzo Di Iorio, Giorgio Palù
Format: Article
Language:English
Published: Elsevier 2018-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118300503
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spelling doaj-9d42870eea36488893ddb39fc14437782020-11-24T22:46:55ZengElsevierStem Cell Research1873-50612018-04-0128149152Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 geneMarta Trevisan0Vanessa Barbaro1Silvia Riccetti2Giulia Masi3Luisa Barzon4Patrizia Nespeca5Gualtiero Alvisi6Enzo Di Iorio7Giorgio Palù8Department of Molecular Medicine, University of Padova, 35121 Padua, Italy; Corresponding author.Fondazione Banca degli Occhi del Veneto, 30174 Venice, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyTransgene free UNIPDi002-A-human induced pluripotent stem cell (hiPSC) line was generated by Sendai Virus Vectors reprogramming from human oral mucosal epithelial stem cells (hOMESCs) of a patient affected by ectrodactyly-ectodermal dysplasia-clefting (EEC)-syndrome, carrying a mutation in exon 8 of the TP63 gene (R304Q). The UNIPDi002-A-hiPSC line retained the mutation of the parental R304Q-hOMESCs and displayed a normal karyotype. No residual expression of transgenes nor Sendai virus vector sequences were detected in the line at passage 8. UNIPDi002-A-hiPSC expressed a panel of pluripotency-associated markers and could form embryoid bodies expressing markers belonging to the three germ layers ectoderm, endoderm and mesoderm.http://www.sciencedirect.com/science/article/pii/S1873506118300503
collection DOAJ
language English
format Article
sources DOAJ
author Marta Trevisan
Vanessa Barbaro
Silvia Riccetti
Giulia Masi
Luisa Barzon
Patrizia Nespeca
Gualtiero Alvisi
Enzo Di Iorio
Giorgio Palù
spellingShingle Marta Trevisan
Vanessa Barbaro
Silvia Riccetti
Giulia Masi
Luisa Barzon
Patrizia Nespeca
Gualtiero Alvisi
Enzo Di Iorio
Giorgio Palù
Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene
Stem Cell Research
author_facet Marta Trevisan
Vanessa Barbaro
Silvia Riccetti
Giulia Masi
Luisa Barzon
Patrizia Nespeca
Gualtiero Alvisi
Enzo Di Iorio
Giorgio Palù
author_sort Marta Trevisan
title Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene
title_short Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene
title_full Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene
title_fullStr Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene
title_full_unstemmed Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene
title_sort generation of a transgene-free induced pluripotent stem cells line (unipdi002-a) from oral mucosa epithelial stem cells carrying the r304q mutation in tp63 gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2018-04-01
description Transgene free UNIPDi002-A-human induced pluripotent stem cell (hiPSC) line was generated by Sendai Virus Vectors reprogramming from human oral mucosal epithelial stem cells (hOMESCs) of a patient affected by ectrodactyly-ectodermal dysplasia-clefting (EEC)-syndrome, carrying a mutation in exon 8 of the TP63 gene (R304Q). The UNIPDi002-A-hiPSC line retained the mutation of the parental R304Q-hOMESCs and displayed a normal karyotype. No residual expression of transgenes nor Sendai virus vector sequences were detected in the line at passage 8. UNIPDi002-A-hiPSC expressed a panel of pluripotency-associated markers and could form embryoid bodies expressing markers belonging to the three germ layers ectoderm, endoderm and mesoderm.
url http://www.sciencedirect.com/science/article/pii/S1873506118300503
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