EFFECTS OF SOME MICHAEL TYPE ADDITION PRODUCTS ON VARIOUS CYTOKINES

• Main Authors: Semra UTKU, Mehtap GÖKÇE, Erdem YEŞİLADA
• Format: Article
• Language: English
• Published: Galenos Yayinevi 2011-04-01
• Series: Türk Yoğun Bakim Derneği Dergisi
• Subjects: Anticytokine agent; Thiobenzoic acid; Thiomethylbenzimidazole; TNF-a
• Online Access: http://www.turkjps.org/archives/archive-detail/article-preview/effects-of-some-mchael-type-addton-products-on-var/12468

The aim of this research in to investigate the anticytokine activities of the 2-[(2-nitro-l-phenylpropyl)thio]benzoic acid (1), 2-[(2-nitro-l-phenylethyl)thiomethyl]benzimidazole (2) and 2-[(2-nitro-l-phenylpropyl)thiomethyl]benzimidazole (3) derivatives in human primary cells and cell lines. Cytokines are messengers for the regulation of the inflammatory cascades with Tumor Necrosis Factor-a (TNF-a), Interleukin (IL-lβ, IL-2, IL-4 andIL-8), Gamma Interferon (IFN-y) working synergistically. In this study which is performed in cell assay, inhibition capacity of compound 1, 2 and 3 derivatives againts TNF-a, IL-Iβ, IL-8, IL-2, IL-4 and IFN-y production by human whole blood have been measured. The test results were shown that 1, 2 and 3 derivatives have dose-dependent inhibitions on the release of IL-1β, IL-8 and TNF-a in lipopolysaccharide (LPS) stimulated human whole blood and IL-2, IL-4 and IFN-yin phorbolacetate (PHA) stimulated in human whole blood