Screening of sodicity tolerant and to survey parental polymorphism in CSR 11 X MI 48 cross using SSR markers

• Main Author: Shveta Bathla, Sahil Bhushan, Ridhi Mehta, Kuldeep and Bineet Kaur
• Format: Article
• Language: English
• Published: Indian Society of Plant Breeders 2010-07-01
• Series: Electronic Journal of Plant Breeding
• Subjects: Rice; sodicity; screening; ssr markers
• Online Access: http://sites.google.com/site/ejpb10/vol-1-4-3/Vol-1-4-1180-1185.pdf?attredirects=0

Rice (Oryza.sativa.L) is adapted to all types of soils. Soils in semi-arid or arid regions, especially those with ineffectivedrainage, accumulate salt as irrigation water evaporates leaving behind deposits of soluble salts. Sodic soil is the one that hasbeen adversely affected by sodium. Sodium toxicity represents the major ionic stress associated with high salinity. Rice canbe grown in alkali soils having pH upto 9.6, but in soils having higher pH, alkali tolerant varieties should be grown or someamendment should be done to reduce the pH upto 9.8.Coexistence of distant genetic forms in population, is actually whatpolymorphism means. Parental polymorphism could be surveyed using SSR markers which are becoming standard DNAmarkers for plant genome analysis and are now being used in marker assisted breeding.DNA extraction and purification ofsodicity tolerance( pH 9.8) CSR11 and MI48 varieties was done. Forty three SSR primers and five saltol SSR primers wereused to screen the parental varieties. Among SSR primers, 26 showed polymorphism between the parents and seventeenwere found to be monomorphic. Among five Saltol AAE primers, two primers showed monomorphism and three showedpolymorphism.