A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaks

• Main Authors: Müller Marcus, Leake Alan, Sindlinger Thilo, Pfeiffer Ragen, Moreno-Villanueva María, Kirkwood Thomas BL, Bürkle Alexander
• Format: Article
• Language: English
• Published: BMC 2009-04-01
• Series: BMC Biotechnology
• Online Access: http://www.biomedcentral.com/1472-6750/9/39

<p>Abstract</p> <p>Background</p> <p>Formation and repair of DNA single-strand breaks are important parameters in the assessment of DNA damage and repair occurring in live cells. The 'Fluorimetric Detection of Alkaline DNA Unwinding (FADU)' method [Birnboim HC, Jevcak JJ. Cancer Res (1981) 41:1889–1892] is a sensitive procedure to quantify DNA strand breaks, yet it is very tedious to perform.</p> <p>Results</p> <p>In order (i) to render the FADU assay more convenient and robust, (ii) to increase throughput, and (iii) to reduce the number of cells needed, we have established a modified assay version that is largely automated and is based on the use of a liquid handling device. The assay is operated in a 96-well format, thus greatly increasing throughput. The number of cells required has been reduced to less than 10,000 per data point. The threshold for detection of X-ray-induced DNA strand breaks is 0.13 Gy. The total assay time required for a typical experiment to assess DNA strand break repair is 4–5 hours.</p> <p>Conclusion</p> <p>We have established a robust and convenient method measuring of formation and repair of DNA single-strand breaks in live cells. While the sensitivity of our method is comparable to current assays, throughput is massively increased while operator time is decreased.</p>