Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.

One of the central questions of developmental biology is how cells of equivalent potential-an equivalence group-come to adopt specific cellular fates. In this study we have used a combination of live imaging, single cell lineage analyses, and perturbation of specific signaling pathways to dissect th...

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Main Authors: Mai E Nguyen-Chi, Robert Bryson-Richardson, Carmen Sonntag, Thomas E Hall, Abigail Gibson, Tamar Sztal, Wendy Chua, Thomas F Schilling, Peter D Currie
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS Genetics
Online Access:http://europepmc.org/articles/PMC3486873?pdf=render
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spelling doaj-9cbd628f20e842fd84861d2dd9fc4bb02020-11-25T02:23:07ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042012-01-01810e100301410.1371/journal.pgen.1003014Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.Mai E Nguyen-ChiRobert Bryson-RichardsonCarmen SonntagThomas E HallAbigail GibsonTamar SztalWendy ChuaThomas F SchillingPeter D CurrieOne of the central questions of developmental biology is how cells of equivalent potential-an equivalence group-come to adopt specific cellular fates. In this study we have used a combination of live imaging, single cell lineage analyses, and perturbation of specific signaling pathways to dissect the specification of the adaxial cells of the zebrafish embryo. We show that the adaxial cells are myogenic precursors that form a cell fate equivalence group of approximately 20 cells that consequently give rise to two distinct sub-types of muscle fibers: the superficial slow muscle fibers (SSFs) and muscle pioneer cells (MPs), distinguished by specific gene expression and cell behaviors. Using a combination of live imaging, retrospective and indicative fate mapping, and genetic studies, we show that MP and SSF precursors segregate at the beginning of segmentation and that they arise from distinct regions along the anterior-posterior (AP) and dorsal-ventral (DV) axes of the adaxial cell compartment. FGF signaling restricts MP cell fate in the anterior-most adaxial cells in each somite, while BMP signaling restricts this fate to the middle of the DV axis. Thus our results reveal that the synergistic actions of HH, FGF, and BMP signaling independently create a three-dimensional (3D) signaling milieu that coordinates cell fate within the adaxial cell equivalence group.http://europepmc.org/articles/PMC3486873?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Mai E Nguyen-Chi
Robert Bryson-Richardson
Carmen Sonntag
Thomas E Hall
Abigail Gibson
Tamar Sztal
Wendy Chua
Thomas F Schilling
Peter D Currie
spellingShingle Mai E Nguyen-Chi
Robert Bryson-Richardson
Carmen Sonntag
Thomas E Hall
Abigail Gibson
Tamar Sztal
Wendy Chua
Thomas F Schilling
Peter D Currie
Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.
PLoS Genetics
author_facet Mai E Nguyen-Chi
Robert Bryson-Richardson
Carmen Sonntag
Thomas E Hall
Abigail Gibson
Tamar Sztal
Wendy Chua
Thomas F Schilling
Peter D Currie
author_sort Mai E Nguyen-Chi
title Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.
title_short Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.
title_full Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.
title_fullStr Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.
title_full_unstemmed Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.
title_sort morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafish myotome.
publisher Public Library of Science (PLoS)
series PLoS Genetics
issn 1553-7390
1553-7404
publishDate 2012-01-01
description One of the central questions of developmental biology is how cells of equivalent potential-an equivalence group-come to adopt specific cellular fates. In this study we have used a combination of live imaging, single cell lineage analyses, and perturbation of specific signaling pathways to dissect the specification of the adaxial cells of the zebrafish embryo. We show that the adaxial cells are myogenic precursors that form a cell fate equivalence group of approximately 20 cells that consequently give rise to two distinct sub-types of muscle fibers: the superficial slow muscle fibers (SSFs) and muscle pioneer cells (MPs), distinguished by specific gene expression and cell behaviors. Using a combination of live imaging, retrospective and indicative fate mapping, and genetic studies, we show that MP and SSF precursors segregate at the beginning of segmentation and that they arise from distinct regions along the anterior-posterior (AP) and dorsal-ventral (DV) axes of the adaxial cell compartment. FGF signaling restricts MP cell fate in the anterior-most adaxial cells in each somite, while BMP signaling restricts this fate to the middle of the DV axis. Thus our results reveal that the synergistic actions of HH, FGF, and BMP signaling independently create a three-dimensional (3D) signaling milieu that coordinates cell fate within the adaxial cell equivalence group.
url http://europepmc.org/articles/PMC3486873?pdf=render
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