Establishment of a Plasmid-Based Reverse Genetics System for the Cell Culture-Adapted Hepatitis E Virus Genotype 3c Strain 47832c

Establishment of a Plasmid-Based Reverse Genetics System for the Cell Culture-Adapted Hepatitis E Virus Genotype 3c Strain 47832c

The hepatitis E virus (HEV) causes acute and chronic hepatitis in humans. Investigation of HEV replication is hampered by the lack of broadly applicable, efficient cell culture systems and tools for site-directed mutagenesis of HEV. The cell culture-adapted genotype 3c strain 47832c, which represent...

Full description

Bibliographic Details
Main Authors:	Johannes Scholz, Christine Bächlein, Ashish K. Gadicherla, Alexander Falkenhagen, Simon H. Tausch, Reimar Johne
Format:	Article
Language:	English
Published:	MDPI AG 2020-02-01
Series:	Pathogens
Subjects:	hepatitis e virus genotype 3c cell culture reverse genetics system site-directed mutagenesis
Online Access:	https://www.mdpi.com/2076-0817/9/3/157

Similar Items

The Translated Amino Acid Sequence of an Insertion in the Hepatitis E Virus Strain 47832c Genome, but Not the RNA Sequence, Is Essential for Efficient Cell Culture Replication
by: Johannes Scholz, et al.
Published: (2021-04-01)

Protein Engineering of Dual-Cys Cyanobacteriochrome AM1_1186g2 for Biliverdin Incorporation and Far-Red/Blue Reversible Photoconversion
by: Yuto Kuwasaki, et al.
Published: (2019-06-01)

Enhanced Replication of Hepatitis E Virus Strain 47832c in an A549-Derived Subclonal Cell Line
by: Mathias Schemmerer, et al.
Published: (2016-09-01)

Generation of Simian Rotavirus Reassortants with VP4- and VP7-Encoding Genome Segments from Human Strains Circulating in Africa Using Reverse Genetics
by: Alexander Falkenhagen, et al.
Published: (2020-02-01)

Studies on the thermostabilization of reverse transcriptases from Moloney murine leukemia virus and avian myeloblastosis virus
by: Konishi, Atsushi
Published: (2015)

Rescue of Infectious Rotavirus Reassortants by a Reverse Genetics System Is Restricted by the Receptor-Binding Region of VP4
by: Alexander Falkenhagen, et al.
Published: (2021-02-01)

Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction
by: Shiho Torii, et al.
Published: (2021-04-01)

Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR
by: Fanli Zeng, et al.
Published: (2017-03-01)

Exciton transfer and trapping in photosystem II
by: Merry, Stephen Alan Paul
Published: (1998)

A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase
by: Kangle Niu, et al.
Published: (2020-08-01)

Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants
by: Marc-André Lemay, et al.
Published: (2019-08-01)

Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance
by: Andries Thangwana, et al.
Published: (2021-01-01)

Hibridização reversa e sequenciamento na genotipagem do vírus da hepatite C Reverse hybridization and sequencing for genotyping the hepatitis C virus
by: Patrícia Martinez Levada, et al.
Published: (2010-04-01)

A novel cold-adapted esterase from Enterobacter cloacae: Characterization and improvement of its activity and thermostability via the site of Tyr193Cys
by: Haofeng Gao, et al.
Published: (2018-03-01)

Development of an Effective and Stable Genotype-Matched Live Attenuated Newcastle Disease Virus Vaccine Based on a Novel Naturally Recombinant Malaysian Isolate Using Reverse Genetics
by: Muhammad Bashir Bello, et al.
Published: (2020-06-01)

A Single Point Mutation, Asn<sub>16</sub>→Lys, Dictates the Temperature-Sensitivity of the Reovirus tsG453 Mutant
by: Kathleen K. M. Glover, et al.
Published: (2021-02-01)

Restriction digest screening facilitates efficient detection of site-directed mutations introduced by CRISPR in C. albicans UME6
by: Ben A. Evans, et al.
Published: (2018-06-01)

Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor
by: Vogel, Walter Kevin
Published: (2012)

The influence of radio-mimetic chemical mutagen on the chromosomal complex of winter wheat cells
by: M. M. Nazarenko
Published: (2017-05-01)

Preferential Protection of Domains II and III of Bacillus thuringiensis Cry1Aa Toxin by Brush Border Membrane Vesicles
by: Syed-Rehan A. Hussain, et al.
Published: (2010-07-01)

Preferential Protection of Domains II and III of Bacillus thuringiensis Cry1Aa Toxin by Brush Border Membrane Vesicles
by: Syed-Rehan A. Hussain, et al.
Published: (2011-01-01)

Enzymatic Properties and Mutational Studies of Chalcone Synthase from <em>Physcomitrella patens</em>
by: Mahiran Basri, et al.
Published: (2012-08-01)

Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis
by: Shuai You, et al.
Published: (2018-06-01)

Effect of induced dNTP pool imbalance on HIV-1 reverse transcription in macrophages
by: Caitlin Shepard, et al.
Published: (2019-10-01)

Mutations in the C-terminal region of the HIV-1 reverse transcriptase and their correlation with drug resistance associated mutations and antiviral treatment
by: Michels I, et al.
Published: (2010-10-01)

Site-directed mutagenesis of yeast V-ATPase subunit d
by: Owegi, Margaret
Published: (2011)

Site-directed mutagenesis of hydrogenase genes in Azotobacter chroococcum
by: Tito, Donald
Published: (1992)

The carboxyl terminus in apolipoprotein E2 and the seven amino acid repeat in apolipoprotein E-Leiden: role in receptor-binding activity
by: Li-Ming Dong, et al.
Published: (1998-06-01)

Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis
by: Shoko Ishibashi, et al.
Published: (2012-10-01)

Mutated Measles Virus Matrix and Fusion Protein Influence Viral Titer In Vitro and Neuro-Invasion in Lewis Rat Brain Slice Cultures
by: Johannes Busch, et al.
Published: (2021-04-01)

Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design
by: Xue-Qing Li, et al.
Published: (2017-05-01)

<it>Chlamydia trachomatis</it> genovar distribution in clinical urogenital specimens from Tunisian patients: high prevalence of <it>C. trachomatis</it> genovar E and mixed infections
by: Gharsallah Houda, et al.
Published: (2012-11-01)

OneClick: A Program for Designing Focused Mutagenesis Experiments
by: Mark Warburton, et al.
Published: (2015-07-01)

LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites
by: Joanna C. Dawes, et al.
Published: (2020-02-01)

Double Mutant Cycles as a Tool to Address Folding, Binding, and Allostery
by: Livia Pagano, et al.
Published: (2021-01-01)

Effect of substitution of Cys11 by Ser on the activity and dimerization of one of rice thioredoxin isoforms (OsTrx23) using site-directed mutagenesis
by: Mitra Roudgar Nashta, et al.
Published: (2015-08-01)

Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440
by: Chunlin Tan, et al.
Published: (2019-01-01)

Development of Therapeutic Chimeric Uricase by Exon Replacement/Restoration and Site-Directed Mutagenesis
by: Guangrong Xie, et al.
Published: (2016-05-01)

Improved immobilization of laccase on a glassy carbon electrode by oriented covalent attachment
by: Liu Xin, et al.
Published: (2014-01-01)

Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile
by: Liu Xin, et al.
Published: (2012-01-01)

Cannot write session to /tmp/vufind_sessions/sess_tr3c2molirogmvmv6cef918p2j