A safety cap protects hydrogenase from oxygen attack

[FeFe]-hydrogenases catalyze the conversion of protons and electrons to molecular hydrogen, but upon exposure to oxygen, their catalytic cofactor is irreversibly inactivated. Here, the authors determine the crystal structure of hydrogenase CbA5H and identify a cysteine residue, which acts as a safet...

Full description

Bibliographic Details
Main Authors:	Martin Winkler, Jifu Duan, Andreas Rutz, Christina Felbek, Lisa Scholtysek, Oliver Lampret, Jan Jaenecke, Ulf-Peter Apfel, Gianfranco Gilardi, Francesca Valetti, Vincent Fourmond, Eckhard Hofmann, Christophe Léger, Thomas Happe
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-02-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-20861-2

id	doaj-9c9745d6547b4e9c8c0781318c4c1e61
record_format	Article
spelling	doaj-9c9745d6547b4e9c8c0781318c4c1e612021-02-07T12:13:46ZengNature Publishing GroupNature Communications2041-17232021-02-0112111010.1038/s41467-020-20861-2A safety cap protects hydrogenase from oxygen attackMartin Winkler0Jifu Duan1Andreas Rutz2Christina Felbek3Lisa Scholtysek4Oliver Lampret5Jan Jaenecke6Ulf-Peter Apfel7Gianfranco Gilardi8Francesca Valetti9Vincent Fourmond10Eckhard Hofmann11Christophe Léger12Thomas Happe13Photobiotechnology, Department of Plant Biochemistry, Ruhr-Universität BochumPhotobiotechnology, Department of Plant Biochemistry, Ruhr-Universität BochumPhotobiotechnology, Department of Plant Biochemistry, Ruhr-Universität BochumCNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la MéditerranéePhotobiotechnology, Department of Plant Biochemistry, Ruhr-Universität BochumPhotobiotechnology, Department of Plant Biochemistry, Ruhr-Universität BochumPhotobiotechnology, Department of Plant Biochemistry, Ruhr-Universität BochumInorganic Chemistry Ι, Department of Chemistry and Biochemistry, Ruhr-Universität BochumDepartment of Life Sciences and Systems Biology, University of TorinoDepartment of Life Sciences and Systems Biology, University of TorinoCNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la MéditerranéeProtein Crystallography, Department of Biophysics, Ruhr-Universität BochumCNRS, Aix-Marseille Université, Laboratoire de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la MéditerranéePhotobiotechnology, Department of Plant Biochemistry, Ruhr-Universität Bochum[FeFe]-hydrogenases catalyze the conversion of protons and electrons to molecular hydrogen, but upon exposure to oxygen, their catalytic cofactor is irreversibly inactivated. Here, the authors determine the crystal structure of hydrogenase CbA5H and identify a cysteine residue, which acts as a safety cap that shields the active site from oxygen.https://doi.org/10.1038/s41467-020-20861-2
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Martin Winkler Jifu Duan Andreas Rutz Christina Felbek Lisa Scholtysek Oliver Lampret Jan Jaenecke Ulf-Peter Apfel Gianfranco Gilardi Francesca Valetti Vincent Fourmond Eckhard Hofmann Christophe Léger Thomas Happe
spellingShingle	Martin Winkler Jifu Duan Andreas Rutz Christina Felbek Lisa Scholtysek Oliver Lampret Jan Jaenecke Ulf-Peter Apfel Gianfranco Gilardi Francesca Valetti Vincent Fourmond Eckhard Hofmann Christophe Léger Thomas Happe A safety cap protects hydrogenase from oxygen attack Nature Communications
author_facet	Martin Winkler Jifu Duan Andreas Rutz Christina Felbek Lisa Scholtysek Oliver Lampret Jan Jaenecke Ulf-Peter Apfel Gianfranco Gilardi Francesca Valetti Vincent Fourmond Eckhard Hofmann Christophe Léger Thomas Happe
author_sort	Martin Winkler
title	A safety cap protects hydrogenase from oxygen attack
title_short	A safety cap protects hydrogenase from oxygen attack
title_full	A safety cap protects hydrogenase from oxygen attack
title_fullStr	A safety cap protects hydrogenase from oxygen attack
title_full_unstemmed	A safety cap protects hydrogenase from oxygen attack
title_sort	safety cap protects hydrogenase from oxygen attack
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-02-01
description	[FeFe]-hydrogenases catalyze the conversion of protons and electrons to molecular hydrogen, but upon exposure to oxygen, their catalytic cofactor is irreversibly inactivated. Here, the authors determine the crystal structure of hydrogenase CbA5H and identify a cysteine residue, which acts as a safety cap that shields the active site from oxygen.
url	https://doi.org/10.1038/s41467-020-20861-2
work_keys_str_mv	AT martinwinkler asafetycapprotectshydrogenasefromoxygenattack AT jifuduan asafetycapprotectshydrogenasefromoxygenattack AT andreasrutz asafetycapprotectshydrogenasefromoxygenattack AT christinafelbek asafetycapprotectshydrogenasefromoxygenattack AT lisascholtysek asafetycapprotectshydrogenasefromoxygenattack AT oliverlampret asafetycapprotectshydrogenasefromoxygenattack AT janjaenecke asafetycapprotectshydrogenasefromoxygenattack AT ulfpeterapfel asafetycapprotectshydrogenasefromoxygenattack AT gianfrancogilardi asafetycapprotectshydrogenasefromoxygenattack AT francescavaletti asafetycapprotectshydrogenasefromoxygenattack AT vincentfourmond asafetycapprotectshydrogenasefromoxygenattack AT eckhardhofmann asafetycapprotectshydrogenasefromoxygenattack AT christopheleger asafetycapprotectshydrogenasefromoxygenattack AT thomashappe asafetycapprotectshydrogenasefromoxygenattack AT martinwinkler safetycapprotectshydrogenasefromoxygenattack AT jifuduan safetycapprotectshydrogenasefromoxygenattack AT andreasrutz safetycapprotectshydrogenasefromoxygenattack AT christinafelbek safetycapprotectshydrogenasefromoxygenattack AT lisascholtysek safetycapprotectshydrogenasefromoxygenattack AT oliverlampret safetycapprotectshydrogenasefromoxygenattack AT janjaenecke safetycapprotectshydrogenasefromoxygenattack AT ulfpeterapfel safetycapprotectshydrogenasefromoxygenattack AT gianfrancogilardi safetycapprotectshydrogenasefromoxygenattack AT francescavaletti safetycapprotectshydrogenasefromoxygenattack AT vincentfourmond safetycapprotectshydrogenasefromoxygenattack AT eckhardhofmann safetycapprotectshydrogenasefromoxygenattack AT christopheleger safetycapprotectshydrogenasefromoxygenattack AT thomashappe safetycapprotectshydrogenasefromoxygenattack
_version_	1724281478900088832

A safety cap protects hydrogenase from oxygen attack

Similar Items