Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications

The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device,...

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Main Authors: Amin Abbaszadeh Banaeiyan, Doryaneh Ahmadpour, Caroline Beck Adiels, Mattias Goksör
Format: Article
Language:English
Published: MDPI AG 2013-12-01
Series:Micromachines
Subjects:
Online Access:http://www.mdpi.com/2072-666X/4/4/414
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spelling doaj-9c9654576cd74729a4de69d3407992072020-11-24T22:58:18ZengMDPI AGMicromachines2072-666X2013-12-014441443010.3390/mi4040414mi4040414Hydrodynamic Cell Trapping for High Throughput Single-Cell ApplicationsAmin Abbaszadeh Banaeiyan0Doryaneh Ahmadpour1Caroline Beck Adiels2Mattias Goksör3Department of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenDepartment of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenDepartment of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenDepartment of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenThe possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min−1, moderate = 50 nL min−1, and high = 100 nL min−1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.http://www.mdpi.com/2072-666X/4/4/414microfluidicssingle cellhigh-throughputhydrodynamic trappingyeastarsenitePDMSfluorescence microscopy
collection DOAJ
language English
format Article
sources DOAJ
author Amin Abbaszadeh Banaeiyan
Doryaneh Ahmadpour
Caroline Beck Adiels
Mattias Goksör
spellingShingle Amin Abbaszadeh Banaeiyan
Doryaneh Ahmadpour
Caroline Beck Adiels
Mattias Goksör
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
Micromachines
microfluidics
single cell
high-throughput
hydrodynamic trapping
yeast
arsenite
PDMS
fluorescence microscopy
author_facet Amin Abbaszadeh Banaeiyan
Doryaneh Ahmadpour
Caroline Beck Adiels
Mattias Goksör
author_sort Amin Abbaszadeh Banaeiyan
title Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
title_short Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
title_full Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
title_fullStr Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
title_full_unstemmed Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
title_sort hydrodynamic cell trapping for high throughput single-cell applications
publisher MDPI AG
series Micromachines
issn 2072-666X
publishDate 2013-12-01
description The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min−1, moderate = 50 nL min−1, and high = 100 nL min−1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.
topic microfluidics
single cell
high-throughput
hydrodynamic trapping
yeast
arsenite
PDMS
fluorescence microscopy
url http://www.mdpi.com/2072-666X/4/4/414
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AT mattiasgoksor hydrodynamiccelltrappingforhighthroughputsinglecellapplications
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