Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device,...
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doaj-9c9654576cd74729a4de69d3407992072020-11-24T22:58:18ZengMDPI AGMicromachines2072-666X2013-12-014441443010.3390/mi4040414mi4040414Hydrodynamic Cell Trapping for High Throughput Single-Cell ApplicationsAmin Abbaszadeh Banaeiyan0Doryaneh Ahmadpour1Caroline Beck Adiels2Mattias Goksör3Department of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenDepartment of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenDepartment of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenDepartment of Physics, University of Gothenburg, Gothenburg, SE-412 96, SwedenThe possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min−1, moderate = 50 nL min−1, and high = 100 nL min−1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.http://www.mdpi.com/2072-666X/4/4/414microfluidicssingle cellhigh-throughputhydrodynamic trappingyeastarsenitePDMSfluorescence microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amin Abbaszadeh Banaeiyan Doryaneh Ahmadpour Caroline Beck Adiels Mattias Goksör |
spellingShingle |
Amin Abbaszadeh Banaeiyan Doryaneh Ahmadpour Caroline Beck Adiels Mattias Goksör Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications Micromachines microfluidics single cell high-throughput hydrodynamic trapping yeast arsenite PDMS fluorescence microscopy |
author_facet |
Amin Abbaszadeh Banaeiyan Doryaneh Ahmadpour Caroline Beck Adiels Mattias Goksör |
author_sort |
Amin Abbaszadeh Banaeiyan |
title |
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications |
title_short |
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications |
title_full |
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications |
title_fullStr |
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications |
title_full_unstemmed |
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications |
title_sort |
hydrodynamic cell trapping for high throughput single-cell applications |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2013-12-01 |
description |
The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min−1, moderate = 50 nL min−1, and high = 100 nL min−1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types. |
topic |
microfluidics single cell high-throughput hydrodynamic trapping yeast arsenite PDMS fluorescence microscopy |
url |
http://www.mdpi.com/2072-666X/4/4/414 |
work_keys_str_mv |
AT aminabbaszadehbanaeiyan hydrodynamiccelltrappingforhighthroughputsinglecellapplications AT doryanehahmadpour hydrodynamiccelltrappingforhighthroughputsinglecellapplications AT carolinebeckadiels hydrodynamiccelltrappingforhighthroughputsinglecellapplications AT mattiasgoksor hydrodynamiccelltrappingforhighthroughputsinglecellapplications |
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