Antibacterial Activity of Benzydamine Hydrochloride against Clinical Isolates of Bacteria, isolated from people in Russia and Spain

• Main Authors: P. V. Slukin, N. K. Fursova, N. I. Briko
• Format: Article
• Language: Russian
• Published: Numikom LLC 2019-01-01
• Series: Эпидемиология и вакцинопрофилактика
• Subjects: benzydamine hydrochloride; antibacterial activity; minimum inhibitory concentration; clinical strains; probiotic strain
• Online Access: https://www.epidemvac.ru/jour/article/view/605
• ISSN: 2073-3046; 2619-0494

Aim to study antibacterial activity of benzydamine hydrochloride, against clinic isolated of bacteria, located in hospitals in Russia and compare this data to results of similar trail of Spanish isolates.Materials  and  method  for this study were used clinical isolates  of bacteria,  which are counted as typical agents  of infections in otorhinolaryngology and gynecology. Enterococcus spp., Escherichia coli, Klebsiella pneumoniae, Staphylococcus spp. and Streptococcus  spp.,  isolated from patients  in Russians  hospitals;  reference  strains  Escherichia coli, Staphylococcus  aureus and Streptococcus agalactiae, from ATCC collection; and also, Lactobacillus acidophilus strain, isolated from probiotic drug «Lactobacterin». Antibacterial activity of benzydamine hydrochloride was evaluated with serial dilution method in agar. For comparison of results we used data of Spanish study of benzydamine activity against clinical isolates and reference strains. The Spanish Study was completed in microbiological department of San-Pau hospital in Barcelona in 2001.Results.  It was indicated that minimum inhibitory concentration (MIC) of benzydamine hydrochloride for clinical isolates of gram-negative and gram-positive  bacteria, isolated from Russian and Spanish hospitals is about the same level: for E. coli it was 640 – 1280  mg/l, for K. pneumoniae  – 512–1280  mg/l, for S. aureus  – 256–1280  mg/l, for S. agalactiae  – 320–1280  mg/l, for S. pyogenes – 256–640 mg/l, for E. faecalis – 512 mg/l, for E. faecium – 256 mg/l, for S. mitis – 640 mg/l, for S. epidermidis 320 – 1280 mg/l, for S. pneumoniae  –  40 mg/l, for S. viridans  –  40 mg/l. The same data was obtained by assessment of sensitivity to benzydamine hydrochloride reference-strains from ATCC collection: for E. coli MIC was 512 – 640 mg/l, for S. aureus – 512 – 640 mg/l, for S. agalactiae – 320 mg/l, for S. pneumoniae – 640 mg/l. Probiotic strain L. acidophilus was resistant to benzydamine hydrochloride activity with MIC = 20000 mg/l. Conclusion: It was indicated that antimicrobial activity of benzydamine hydrochloride against clinical strains, isolated from the patients of hospitals in Russia and Spain. Also, resistance of probiotic strain of lactobacteria was detected to this drug, which indicates the possibility of benzydamine hydrochloride application in clinical practice in otorhinolaryngology  and gynecology without risk of negative influence on normal lactobacterial flora.