A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays
Many current immunoassays require multiple washing, incubation and optimization steps. Here the authors present Ratiometric Plug-and-Play Immunodiagnostics (RAPPID), a generic assay platform that uses ratiometric bioluminescent detection to allow sandwich immunoassays to be performed directly in sol...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2021-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-24874-3 |
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doaj-9c6f00283d7b49f6a84e253e1ef16ce12021-08-01T11:37:25ZengNature Publishing GroupNature Communications2041-17232021-07-0112111210.1038/s41467-021-24874-3A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassaysYan Ni0Bas J. H. M. Rosier1Eva A. van Aalen2Eva T. L. Hanckmann3Lieuwe Biewenga4Anna-Maria Makri Pistikou5Bart Timmermans6Chris Vu7Sophie Roos8Remco Arts9Wentao Li10Tom F. A. de Greef11Marcel M. G. J. van Borren12Frank J. M. van Kuppeveld13Berend-Jan Bosch14Maarten Merkx15Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyInstitute for Complex Molecular Systems, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyVirology Section, Infectious Diseases and Immunology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyDepartment of Clinical Chemistry, Rijnstate HospitalVirology Section, Infectious Diseases and Immunology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityVirology Section, Infectious Diseases and Immunology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of TechnologyMany current immunoassays require multiple washing, incubation and optimization steps. Here the authors present Ratiometric Plug-and-Play Immunodiagnostics (RAPPID), a generic assay platform that uses ratiometric bioluminescent detection to allow sandwich immunoassays to be performed directly in solution.https://doi.org/10.1038/s41467-021-24874-3 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yan Ni Bas J. H. M. Rosier Eva A. van Aalen Eva T. L. Hanckmann Lieuwe Biewenga Anna-Maria Makri Pistikou Bart Timmermans Chris Vu Sophie Roos Remco Arts Wentao Li Tom F. A. de Greef Marcel M. G. J. van Borren Frank J. M. van Kuppeveld Berend-Jan Bosch Maarten Merkx |
| spellingShingle |
Yan Ni Bas J. H. M. Rosier Eva A. van Aalen Eva T. L. Hanckmann Lieuwe Biewenga Anna-Maria Makri Pistikou Bart Timmermans Chris Vu Sophie Roos Remco Arts Wentao Li Tom F. A. de Greef Marcel M. G. J. van Borren Frank J. M. van Kuppeveld Berend-Jan Bosch Maarten Merkx A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays Nature Communications |
| author_facet |
Yan Ni Bas J. H. M. Rosier Eva A. van Aalen Eva T. L. Hanckmann Lieuwe Biewenga Anna-Maria Makri Pistikou Bart Timmermans Chris Vu Sophie Roos Remco Arts Wentao Li Tom F. A. de Greef Marcel M. G. J. van Borren Frank J. M. van Kuppeveld Berend-Jan Bosch Maarten Merkx |
| author_sort |
Yan Ni |
| title |
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays |
| title_short |
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays |
| title_full |
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays |
| title_fullStr |
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays |
| title_full_unstemmed |
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays |
| title_sort |
plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2021-07-01 |
| description |
Many current immunoassays require multiple washing, incubation and optimization steps. Here the authors present Ratiometric Plug-and-Play Immunodiagnostics (RAPPID), a generic assay platform that uses ratiometric bioluminescent detection to allow sandwich immunoassays to be performed directly in solution. |
| url |
https://doi.org/10.1038/s41467-021-24874-3 |
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