Characterization and Optimization of PLA Stereocomplexed Hydrogels for Local Gene Delivery Systems
Localized gene delivery still remains as a challenging therapeutic method due to the multiple hurdles to overcome. One of the significant factors is a development of a matrix to carry and safely deliver genes at the local site in a controlled manner and then exit and disintegrate harmlessly. This re...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-05-01
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| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/11/5/796 |
| Summary: | Localized gene delivery still remains as a challenging therapeutic method due to the multiple hurdles to overcome. One of the significant factors is a development of a matrix to carry and safely deliver genes at the local site in a controlled manner and then exit and disintegrate harmlessly. This report describes the structural and mechanistic studies on the in-situ forming hydrogels composed of the PEI/DNA multi-layered micelles to apply for gene therapy. The stereocomplexation-driven hydrogel systems from the DNA-loaded and DNA-free PLA-PEG-PLA triblock copolymer micelles that include enantiomeric polylactide blocks exhibited a sol-to-gel transitions between room and body temperatures. These hydrogels have well-described structure and compositions, and improved mechanical properties. Furthermore, the investigation of their degradation profiles and chemical analysis indicated the faster acidic degradation and stepwise degradation process of these micelle−hydrogel systems. |
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| ISSN: | 2073-4360 |