qMrdd2, a novel quantitative resistance locus for maize rough dwarf disease

• Main Authors: Weixiao Zhang, Suining Deng, Yan Zhao, Wei Xu, Qingcai Liu, Yongzhong Zhang, Chunmei Ren, Zhaobang Cheng, Mingliang Xu, Baoshen Liu
• Format: Article
• Language: English
• Published: BMC 2021-06-01
• Series: BMC Plant Biology
• Subjects: Maize; MRDD; QTL; Fine-mapping; MAS
• Online Access: https://doi.org/10.1186/s12870-021-03107-1

Abstract Background Maize rough dwarf disease (MRDD), a widespread disease caused by four pathogenic viruses, severely reduces maize yield and grain quality. Resistance against MRDD is a complex trait that controlled by many quantitative trait loci (QTL) and easily influenced by environmental conditions. So far, many studies have reported numbers of resistant QTL, however, only one QTL have been cloned, so it is especially important to map and clone more genes that confer resistance to MRDD. Results In the study, a major quantitative trait locus (QTL) qMrdd2, which confers resistance to MRDD, was identified and fine mapped. qMrdd2, located on chromosome 2, was consistently identified in a 15-Mb interval between the simple sequence repeat (SSR) markers D184 and D1600 by using a recombinant inbred line (RIL) population derived from a cross between resistant (“80007”) and susceptible (“80044”) inbred lines. Using a recombinant-derived progeny test strategy, qMrdd2 was delineated to an interval of 577 kb flanked by markers N31 and N42. We further demonstrated that qMrdd2 is an incompletely dominant resistance locus for MRDD that reduced the disease severity index by 20.4%. Conclusions A major resistance QTL (qMrdd2) have been identified and successfully refined into 577 kb region. This locus will be valuable for improving maize variety resistance to MRDD via marker-assisted selection (MAS).