Antimicrobial activity of some essential oils against oral multidrug–resistant Enterococcus faecalis in both planktonic and biofilm state

• Main Authors: Fethi Benbelaïd, Abdelmounaïm Khadir, Mohamed Amine Abdoune, Mourad Bendahou, Alain Muselli, Jean Costa
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014-06-01
• Series: Asian Pacific Journal of Tropical Biomedicine
• Subjects: Bacterial infections; Biofilm; Enterococcus faecalis; Essential oils; Multidrug-resistance
• Online Access: http://www.sciencedirect.com/science/article/pii/S2221169115302240

Objective: To evaluate some essential oils in treatment of intractable oral infections, principally caused by biofilm of multidrug-resistant Enterococcus faecalis (E. faecalis), such as persistent endodontic infections in which their treatment exhibits a real challenge for dentists. Methods: Ten chemically analyzed essential oils by gas chromatography-mass spectrometry were evaluated for antimicrobial activity against sensitive and resistant clinical strains of E. faecalis in both planktonic and biofilm state using two methods, disk diffusion and broth micro-dilution. Results: Studied essential oils showed a good antimicrobial activity and high ability in E. faecalis biofilm eradication, whether for sensitive or multidrug-resistant strains, especially those of Origanum glandulosum and Thymbra capitata with interesting minimum inhibitory concentration, biofilm inhibitory concentration, and biofilm eradication concentration values which doesn't exceed 0.063%, 0.75%, and 1.5%, respectively. Conclusions: Findings of this study indicate that essential oils extracted from aromatic plants can be used in treatment of intractable oral infections, especially caused by biofilm of multidrug-resistant E. faecalis.