Clinical Value of S-poly (T) plus Real-time PCR in Detection of Liver Cancer-related miR-199

• Main Authors: WU Juan, ZHANG Yaqin, WU Yunbin, CHEN Min, YU Zhou, CAO Yingping
• Format: Article
• Language: zho
• Published: Magazine House of Cancer Research on Prevention and Treatment 2020-10-01
• Series: Zhongliu Fangzhi Yanjiu
• Subjects: liver cancer; qrt-pcr; mir-199; s-poly (t) plus qrt-pcr method; poly (a) qrt-pcr method
• Online Access: http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.1618.htm

Objective To explore the advantages and disadvantages of S-poly(T) plus qRT-PCR and Poly (A) qRT-PCR methods in detecting peripheral blood serum miR-199. Methods S-poly(T) plus RT-PCR and Poly(A) qRT-PCR methods were used to detect the serum miR-199 in patients with liver cancer and healthy controls in three aspects of sensitivity, linearity and repeatability to evaluate their potential clinical value. Results The Ct values of S-poly(T) plus qRT-PCR method for miR-199 in healthy controls was significantly lower than that of Poly(A) qRT-PCR method (P < 0.0001); and the S-poly(T) plus qRT-PCR method had better linear correlation (P=0.0027) and repeatability (P=0.010). S-poly(T) plus qRT-PCR method had better sensitivity in detecting serum miR-199 in liver cancer patients comparing with Poly (A) qRT-PCR method. Conclusion In the detection of liver cancer serum miR-199, S-poly(T) plus qRT-PCR method is more sensitive, repeatable and stable than Poly(A) qRT-PCR.