A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation

A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation

MoS2 nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS2 nanosheets and restriction endonuclease BstUI for methylation analysis of p16 pr...

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Main Authors: Le Xiao, Li Xu, Chuan Gao, Yulin Zhang, Qunfeng Yao, Guo-Jun Zhang
Format: Article
Language:English
Published: MDPI AG 2016-09-01
Series:Sensors
Subjects:
MoS2
fluorescence biosensor
homogeneous analysis
DNA methylation
Online Access:http://www.mdpi.com/1424-8220/16/10/1561
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spelling doaj-9c2bbd3835204b10a4fef602095b9feb2020-11-24T23:40:56ZengMDPI AGSensors1424-82202016-09-011610156110.3390/s16101561s16101561A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA MethylationLe Xiao0Li Xu1Chuan Gao2Yulin Zhang3Qunfeng Yao4Guo-Jun Zhang5School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Pharmacy, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaMoS2 nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS2 nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance.http://www.mdpi.com/1424-8220/16/10/1561MoS2fluorescence biosensorhomogeneous analysisDNA methylation
collection DOAJ
language English
format Article
sources DOAJ
author Le Xiao
Li Xu
Chuan Gao
Yulin Zhang
Qunfeng Yao
Guo-Jun Zhang
spellingShingle Le Xiao
Li Xu
Chuan Gao
Yulin Zhang
Qunfeng Yao
Guo-Jun Zhang
A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
Sensors
MoS2
fluorescence biosensor
homogeneous analysis
DNA methylation
author_facet Le Xiao
Li Xu
Chuan Gao
Yulin Zhang
Qunfeng Yao
Guo-Jun Zhang
author_sort Le Xiao
title A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_short A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_full A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_fullStr A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_full_unstemmed A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
title_sort mos2 nanosheet-based fluorescence biosensor for simple and quantitative analysis of dna methylation
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2016-09-01
description MoS2 nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS2 nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance.
topic MoS2
fluorescence biosensor
homogeneous analysis
DNA methylation
url http://www.mdpi.com/1424-8220/16/10/1561
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