BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities

The failure of current universal taxonomic databases to support the rapidly expanding field of bee microbiota research has led to many investigators relying on “in-house” reference sets or manual classification of sequence reads (usually based on BLAST searches), often with vague identity...

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Main Authors: Brendan A. Daisley, Gregor Reid
Format: Article
Language:English
Published: American Society for Microbiology 2021-04-01
Series:mSystems
Online Access:https://journals.asm.org/doi/10.1128/mSystems.00082-21
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spelling doaj-9c14296e762641a5a6a0c0ef3589a7c02021-09-21T20:42:23ZengAmerican Society for MicrobiologymSystems2379-50772021-04-016210.1128/mSystems.00082-21BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial CommunitiesBrendan A. Daisley0https://orcid.org/0000-0001-5999-5792Gregor Reid1https://orcid.org/0000-0001-9658-5696Department of Microbiology & Immunology, The University of Western Ontario, London, Ontario, CanadaDepartment of Microbiology & Immunology, The University of Western Ontario, London, Ontario, Canada The failure of current universal taxonomic databases to support the rapidly expanding field of bee microbiota research has led to many investigators relying on “in-house” reference sets or manual classification of sequence reads (usually based on BLAST searches), often with vague identity thresholds and subjective taxonomy choices. This time expensive, error- and bias-prone process lacks standardization, cripples the potential for comparative cross-study analysis, and in many cases is likely to sway study conclusions.https://journals.asm.org/doi/10.1128/mSystems.00082-21
collection DOAJ
language English
format Article
sources DOAJ
author Brendan A. Daisley
Gregor Reid
spellingShingle Brendan A. Daisley
Gregor Reid
BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities
mSystems
author_facet Brendan A. Daisley
Gregor Reid
author_sort Brendan A. Daisley
title BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities
title_short BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities
title_full BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities
title_fullStr BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities
title_full_unstemmed BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities
title_sort beexact: a metataxonomic database tool for high-resolution inference of bee-associated microbial communities
publisher American Society for Microbiology
series mSystems
issn 2379-5077
publishDate 2021-04-01
description The failure of current universal taxonomic databases to support the rapidly expanding field of bee microbiota research has led to many investigators relying on “in-house” reference sets or manual classification of sequence reads (usually based on BLAST searches), often with vague identity thresholds and subjective taxonomy choices. This time expensive, error- and bias-prone process lacks standardization, cripples the potential for comparative cross-study analysis, and in many cases is likely to sway study conclusions.
url https://journals.asm.org/doi/10.1128/mSystems.00082-21
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