BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities
The failure of current universal taxonomic databases to support the rapidly expanding field of bee microbiota research has led to many investigators relying on “in-house” reference sets or manual classification of sequence reads (usually based on BLAST searches), often with vague identity...
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American Society for Microbiology
2021-04-01
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doaj-9c14296e762641a5a6a0c0ef3589a7c02021-09-21T20:42:23ZengAmerican Society for MicrobiologymSystems2379-50772021-04-016210.1128/mSystems.00082-21BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial CommunitiesBrendan A. Daisley0https://orcid.org/0000-0001-5999-5792Gregor Reid1https://orcid.org/0000-0001-9658-5696Department of Microbiology & Immunology, The University of Western Ontario, London, Ontario, CanadaDepartment of Microbiology & Immunology, The University of Western Ontario, London, Ontario, Canada The failure of current universal taxonomic databases to support the rapidly expanding field of bee microbiota research has led to many investigators relying on “in-house” reference sets or manual classification of sequence reads (usually based on BLAST searches), often with vague identity thresholds and subjective taxonomy choices. This time expensive, error- and bias-prone process lacks standardization, cripples the potential for comparative cross-study analysis, and in many cases is likely to sway study conclusions.https://journals.asm.org/doi/10.1128/mSystems.00082-21 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Brendan A. Daisley Gregor Reid |
spellingShingle |
Brendan A. Daisley Gregor Reid BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities mSystems |
author_facet |
Brendan A. Daisley Gregor Reid |
author_sort |
Brendan A. Daisley |
title |
BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities |
title_short |
BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities |
title_full |
BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities |
title_fullStr |
BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities |
title_full_unstemmed |
BEExact: a Metataxonomic Database Tool for High-Resolution Inference of Bee-Associated Microbial Communities |
title_sort |
beexact: a metataxonomic database tool for high-resolution inference of bee-associated microbial communities |
publisher |
American Society for Microbiology |
series |
mSystems |
issn |
2379-5077 |
publishDate |
2021-04-01 |
description |
The failure of current universal taxonomic databases to support the rapidly expanding field of bee microbiota research has led to many investigators relying on “in-house” reference sets or manual classification of sequence reads (usually based on BLAST searches), often with vague identity thresholds and subjective taxonomy choices. This time expensive, error- and bias-prone process lacks standardization, cripples the potential for comparative cross-study analysis, and in many cases is likely to sway study conclusions. |
url |
https://journals.asm.org/doi/10.1128/mSystems.00082-21 |
work_keys_str_mv |
AT brendanadaisley beexactametataxonomicdatabasetoolforhighresolutioninferenceofbeeassociatedmicrobialcommunities AT gregorreid beexactametataxonomicdatabasetoolforhighresolutioninferenceofbeeassociatedmicrobialcommunities |
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1717372114280906752 |