EFFECT OF ETHANOL ON RESPIRATION AND PHOTOSYNTHESIS OF EUGLENA GRACILIS

• Main Authors: В. М. Мокросноп, О. В. Поліщук, О. К. Золотарьова
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2014-09-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: euglena gracilis; фотосинтез; дихання; етанол; варіабельна флуоресценція хлорофілу
• Online Access: http://mbt.onu.edu.ua/article/view/48291

The aim of the present work was to study the effect of ethanol on the rates of photosynthesis and respiration, and productivity of Euglena gracilis. Methods.The growth rate of culture was determined by cell concentration. Dark respiration and net photosynthesis were measured by O2 concentration changes with a Clark-type electrode. Photosynthesis efficiency was also determined as effective quantum yield of PSII with Xe-PAM fluorometer (Walz, Germany). Results. Addition of 100 mM ethanol significantly stimulated the growth of light-adapted E. gracilis. At the same time, respiration rate tripled and light-dependent oxygen evolution rate doubled. Methanol caused no significant changes. Conclusions. Cultivated on the light E. gracilis effectively assimilated ethyl alcohol, that was accompanied by photosynthesis activation and culture biomass accumulation.