The effect of hydro-alcoholic Prosopis farcta fruit extract on blood glucose and gene expression of pyruvate kinase in type 1 diabetic rats

• Main Authors: Mosa Darvish Sargazi, Sadigh Sabbagh, Hamid reza Miri, SHala Najafi, Kazem Sabbagh
• Format: Article
• Language: fas
• Published: Lorestan University of Medical Science 2016-02-01
• Series: Yafteh
• Subjects: Type 1 diabete; Prosopis farcta; rat; Pyruvate kinase
• Online Access: http://yafte.lums.ac.ir/browse.php?a_code=A-10-1693-6&slc_lang=en&sid=1

Background: With respect to antioxidant effect of Prosopis farcta fruit extract and the role of antioxidant agents in diabetes improvement, the aim of this study was to investigate the effect of hydro-alcoholic Prosopis farcta fruit extract on blood glucose and gene expression of pyruvate kinase (PK). Materials and Methods: Type 1 diabete was induced in male wistar rats (150-300 g) by injection of 60 mg/kg of streptozotocin. Diabetic group received 300 mg/kg of Prosopis farcta fruit extract daily 30 days. The group of normal control and diabetic control received distilled water. Then the glucose level was investigated in day before injection and on days 15 and 30 after injection of prosopis farcta fruit extract. PK gene expression of liver tissue was analysed by Real-Time PCR. Results: The result showed the blood glucose compare to diabetic control significantly decreased on days 15 and there was not any significant between diabetic control. The result of PK gene expression showed that the gene expression in diabetic treatment group compared to diabetic control increased on day 15 and then the expression did not change on day 30 but still wars greater than diabetic control. Conclusion: The result of this study showed that hydro-alcoholic prosopis farcta fruit extract could probably decrease blood glucose by increasing PK gene expression.