DEVELOPMENT AND BIOLOGICAL TESTING OF NEW SCAFFOLDS FROM BIODEGRADABLE MATERIALS

• Main Authors: V. N. Vasilets, L. M. Zaitov, A. Y. Milentev, S. L. Nedoseev, P. V. Shvartskopf, V. A. Egorova, V. I. Sevastjanov
• Format: Article
• Language: Russian
• Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 2010-06-01
• Series: Vestnik Transplantologii i Iskusstvennyh Organov
• Subjects: cell technologies; biodegradable scaffolds; poly(hydroxybutyrate-co-hydroxyvalerate); gel- sublimation technique; gas discharge plasma; hemolysis; cytotoxicity
• Online Access: https://journal.transpl.ru/vtio/article/view/309

The new gel-sublimation technique for preparation porus biodegradable scaffolds (hydroxybutyrate-co-hydro- xyvalerate) is presented. The scaffolds with multi-mode internal structure have the of porus sizes varied from ~100 microns up to ~100 nanometers and the porosity in a range of 80–90%. A few techniques for modification of 3D scaffolds by gas discharge plasma are developed and optimized: the microsecond dielectric barrier dischar- ge, the semi-self-maintained discharge supported by an electron beam; the nanosecond dielectric barrier dischar- ge. Biological tests including red blood cell hemolysis and cytotoxicity analysis have shown the possibilities of scaffolds applications for cell-based technologies.