Huntingtin gene evolution in Chordata and its peculiar features in the ascidian <it>Ciona </it>genus

• Main Authors: Cattaneo Elena, Pesole Graziano, Gissi Carmela, Tartari Marzia
• Format: Article
• Language: English
• Published: BMC 2006-11-01
• Series: BMC Genomics
• Online Access: http://www.biomedcentral.com/1471-2164/7/288

<p>Abstract</p> <p>Background</p> <p>To gain insight into the evolutionary features of the huntingtin (htt) gene in Chordata, we have sequenced and characterized the full-length htt mRNA in the ascidian <it>Ciona intestinalis</it>, a basal chordate emerging as new invertebrate model organism. Moreover, taking advantage of the availability of genomic and EST sequences, the htt gene structure of a number of chordate species, including the cogeneric ascidian <it>Ciona savignyi</it>, and the vertebrates <it>Xenopus </it>and <it>Gallus </it>was reconstructed.</p> <p>Results</p> <p>The <it>C. intestinalis </it>htt transcript exhibits some peculiar features, such as spliced leader trans-splicing in the 98 nt-long 5' untranslated region (UTR), an alternative splicing in the coding region, eight alternative polyadenylation sites, and no similarities of both 5' and 3'UTRs compared to homologs of the cogeneric <it>C. savignyi</it>. The predicted protein is 2946 amino acids long, shorter than its vertebrate homologs, and lacks the polyQ and the polyP stretches found in the the N-terminal regions of mammalian homologs. The exon-intron organization of the htt gene is almost identical among vertebrates, and significantly conserved between <it>Ciona </it>and vertebrates, allowing us to hypothesize an ancestral chordate gene consisting of at least 40 coding exons.</p> <p>Conclusion</p> <p>During chordate diversification, events of gain/loss, sliding, phase changes, and expansion of introns occurred in both vertebrate and ascidian lineages predominantly in the 5'-half of the htt gene, where there is also evidence of lineage-specific evolutionary dynamics in vertebrates. On the contrary, the 3'-half of the gene is highly conserved in all chordates at the level of both gene structure and protein sequence. Between the two <it>Ciona </it>species, a fast evolutionary rate and/or an early divergence time is suggested by the absence of significant similarity between UTRs, protein divergence comparable to that observed between mammals and fishes, and different distribution of repetitive elements.</p>