<it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells
<p>Abstract</p> <p>Background</p> <p>Although fungi have been implicated as initiating/deteriorating factors for allergic asthma, their contributing components have not been fully elucidated. We previously isolated soluble β-glucan from <it>Candida albicans </i...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2009-07-01
|
Series: | Respiratory Research |
Online Access: | http://respiratory-research.com/content/10/1/68 |
id |
doaj-9bc2449f101743f783e6d61f97827d28 |
---|---|
record_format |
Article |
spelling |
doaj-9bc2449f101743f783e6d61f97827d282020-11-24T20:44:16ZengBMCRespiratory Research1465-99212009-07-011016810.1186/1465-9921-10-68<it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cellsTamura HiroshiAdachi YoshiyukiOda ToshioYanagisawa RieKoike EikoTakano HirohisaInoue Ken-ichiroIshibashi Ken-ichiOhno Naohito<p>Abstract</p> <p>Background</p> <p>Although fungi have been implicated as initiating/deteriorating factors for allergic asthma, their contributing components have not been fully elucidated. We previously isolated soluble β-glucan from <it>Candida albicans </it>(CSBG) (Ohno et al., 2007). In the present study, the effects of CSBG exposure on airway immunopathology in the presence or absence of other immunogenic allergen was investigated <it>in vivo</it>, and their cellular mechanisms were analyzed both <it>in vivo </it>and <it>in vitro</it>.</p> <p>Methods</p> <p><it>In vivo</it>, ICR mice were divided into 4 experimental groups: vehicle, CSBG (25 μg/animal), ovalbumin (OVA: 2 μg/animal), and CSBG + OVA were repeatedly administered intratracheally. The bronchoalveolar lavage cellular profile, lung histology, levels of cytokines and chemokines in the lung homogenates, the expression pattern of antigen-presenting cell (APC)-related molecules in the lung digests, and serum immunoglobulin values were studied. <it>In vitro</it>, the impacts of CSBG (0–12.5 μg/ml) on the phenotype and function of immune cells such as splenocytes and bone marrow-derived dendritic cells (BMDCs) were evaluated in terms of cell proliferation, the surface expression of APC-related molecules, and OVA-mediated T-cell proliferating activity.</p> <p>Results</p> <p><it>In vivo</it>, repeated pulmonary exposure to CSBG induced neutrophilic airway inflammation in the absence of OVA, and markedly exacerbated OVA-related eosinophilic airway inflammation with mucus metaplasia in mice, which was concomitant with the amplified lung expression of Th2 cytokines and IL-17A and chemokines related to allergic response. Exposure to CSBG plus OVA increased the number of cells bearing MHC class II with or without CD80 in the lung compared to that of others. <it>In vitro</it>, CSBG significantly augmented splenocyte proliferation in the presence or absence of OVA. Further, CSBG increased the expression of APC-related molecules such as CD80, CD86, and DEC205 on BMDCs and amplified OVA-mediated T-cell proliferation through BMDCs.</p> <p>Conclusion</p> <p>CSBG potentiates allergic airway inflammation with maladaptive Th immunity, and this potentiation was associated with the enhanced activation of APCs including DC.</p> http://respiratory-research.com/content/10/1/68 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tamura Hiroshi Adachi Yoshiyuki Oda Toshio Yanagisawa Rie Koike Eiko Takano Hirohisa Inoue Ken-ichiro Ishibashi Ken-ichi Ohno Naohito |
spellingShingle |
Tamura Hiroshi Adachi Yoshiyuki Oda Toshio Yanagisawa Rie Koike Eiko Takano Hirohisa Inoue Ken-ichiro Ishibashi Ken-ichi Ohno Naohito <it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells Respiratory Research |
author_facet |
Tamura Hiroshi Adachi Yoshiyuki Oda Toshio Yanagisawa Rie Koike Eiko Takano Hirohisa Inoue Ken-ichiro Ishibashi Ken-ichi Ohno Naohito |
author_sort |
Tamura Hiroshi |
title |
<it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells |
title_short |
<it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells |
title_full |
<it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells |
title_fullStr |
<it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells |
title_full_unstemmed |
<it>Candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells |
title_sort |
<it>candida </it>soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: possible role of antigen-presenting cells |
publisher |
BMC |
series |
Respiratory Research |
issn |
1465-9921 |
publishDate |
2009-07-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Although fungi have been implicated as initiating/deteriorating factors for allergic asthma, their contributing components have not been fully elucidated. We previously isolated soluble β-glucan from <it>Candida albicans </it>(CSBG) (Ohno et al., 2007). In the present study, the effects of CSBG exposure on airway immunopathology in the presence or absence of other immunogenic allergen was investigated <it>in vivo</it>, and their cellular mechanisms were analyzed both <it>in vivo </it>and <it>in vitro</it>.</p> <p>Methods</p> <p><it>In vivo</it>, ICR mice were divided into 4 experimental groups: vehicle, CSBG (25 μg/animal), ovalbumin (OVA: 2 μg/animal), and CSBG + OVA were repeatedly administered intratracheally. The bronchoalveolar lavage cellular profile, lung histology, levels of cytokines and chemokines in the lung homogenates, the expression pattern of antigen-presenting cell (APC)-related molecules in the lung digests, and serum immunoglobulin values were studied. <it>In vitro</it>, the impacts of CSBG (0–12.5 μg/ml) on the phenotype and function of immune cells such as splenocytes and bone marrow-derived dendritic cells (BMDCs) were evaluated in terms of cell proliferation, the surface expression of APC-related molecules, and OVA-mediated T-cell proliferating activity.</p> <p>Results</p> <p><it>In vivo</it>, repeated pulmonary exposure to CSBG induced neutrophilic airway inflammation in the absence of OVA, and markedly exacerbated OVA-related eosinophilic airway inflammation with mucus metaplasia in mice, which was concomitant with the amplified lung expression of Th2 cytokines and IL-17A and chemokines related to allergic response. Exposure to CSBG plus OVA increased the number of cells bearing MHC class II with or without CD80 in the lung compared to that of others. <it>In vitro</it>, CSBG significantly augmented splenocyte proliferation in the presence or absence of OVA. Further, CSBG increased the expression of APC-related molecules such as CD80, CD86, and DEC205 on BMDCs and amplified OVA-mediated T-cell proliferation through BMDCs.</p> <p>Conclusion</p> <p>CSBG potentiates allergic airway inflammation with maladaptive Th immunity, and this potentiation was associated with the enhanced activation of APCs including DC.</p> |
url |
http://respiratory-research.com/content/10/1/68 |
work_keys_str_mv |
AT tamurahiroshi itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT adachiyoshiyuki itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT odatoshio itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT yanagisawarie itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT koikeeiko itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT takanohirohisa itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT inouekenichiro itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT ishibashikenichi itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells AT ohnonaohito itcandidaitsolublecellwallbglucanfacilitatesovalbumininducedallergicairwayinflammationinmicepossibleroleofantigenpresentingcells |
_version_ |
1716817896290648064 |