A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis.
Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living an...
Main Authors: | Kedong Xu, Xiaohui Huang, Manman Wu, Yan Wang, Yunxia Chang, Kun Liu, Ju Zhang, Yi Zhang, Fuli Zhang, Liming Yi, Tingting Li, Ruiyue Wang, Guangxuan Tan, Chengwei Li |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
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Series: | PLoS ONE |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416168/pdf/?tool=EBI |
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