ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.

Post-operative surgical site infections (SSI) present a serious threat and may lead to complications. Currently available dressings for SSI lack mucoadhesion, safety, efficacy and most importantly patient compliance. We aimed to address these concerns by developing a bioactive thiolated chitosan-alg...

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Main Authors: Rabia Arshad, Muhammad Farhan Sohail, Hafiz Shoaib Sarwar, Hamid Saeed, Imran Ali, Sohail Akhtar, Syed Zajif Hussain, Iqra Afzal, Sarwat Jahan, Anees-Ur-Rehman, Gul Shahnaz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0217079
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spelling doaj-9b9f7af21b4c479f9ca2cb5c3826dfa92021-03-03T20:38:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01146e021707910.1371/journal.pone.0217079ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.Rabia ArshadMuhammad Farhan SohailHafiz Shoaib SarwarHamid SaeedImran AliSohail AkhtarSyed Zajif HussainIqra AfzalSarwat JahanAnees-Ur-RehmanGul ShahnazPost-operative surgical site infections (SSI) present a serious threat and may lead to complications. Currently available dressings for SSI lack mucoadhesion, safety, efficacy and most importantly patient compliance. We aimed to address these concerns by developing a bioactive thiolated chitosan-alginate bandage embedded with zinc oxide nanoparticles (ZnO-NPs) for localized topical treatment of SSI. The FTIR, XRD, DSC and TGA of bandage confirmed the compatibility of ingredients and modifications made. The porosity, swelling index and lysozyme degradation showed good properties for wound healing and biodegradation. Moreover, in-vitro antibacterial activity showed higher bactericidal effect as compared to ZnO-NPs free bandage. In-vivo wound healing in murine model showed significant improved tissue generation and speedy wound healing as compared to positive and negative controls. Over all, thiolated bandage showed potential as an advanced therapeutic agent for treating surgical site infections, meeting the required features of an ideal surgical dressing.https://doi.org/10.1371/journal.pone.0217079
collection DOAJ
language English
format Article
sources DOAJ
author Rabia Arshad
Muhammad Farhan Sohail
Hafiz Shoaib Sarwar
Hamid Saeed
Imran Ali
Sohail Akhtar
Syed Zajif Hussain
Iqra Afzal
Sarwat Jahan
Anees-Ur-Rehman
Gul Shahnaz
spellingShingle Rabia Arshad
Muhammad Farhan Sohail
Hafiz Shoaib Sarwar
Hamid Saeed
Imran Ali
Sohail Akhtar
Syed Zajif Hussain
Iqra Afzal
Sarwat Jahan
Anees-Ur-Rehman
Gul Shahnaz
ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.
PLoS ONE
author_facet Rabia Arshad
Muhammad Farhan Sohail
Hafiz Shoaib Sarwar
Hamid Saeed
Imran Ali
Sohail Akhtar
Syed Zajif Hussain
Iqra Afzal
Sarwat Jahan
Anees-Ur-Rehman
Gul Shahnaz
author_sort Rabia Arshad
title ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.
title_short ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.
title_full ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.
title_fullStr ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.
title_full_unstemmed ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation.
title_sort zno-nps embedded biodegradable thiolated bandage for postoperative surgical site infection: in vitro and in vivo evaluation.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2019-01-01
description Post-operative surgical site infections (SSI) present a serious threat and may lead to complications. Currently available dressings for SSI lack mucoadhesion, safety, efficacy and most importantly patient compliance. We aimed to address these concerns by developing a bioactive thiolated chitosan-alginate bandage embedded with zinc oxide nanoparticles (ZnO-NPs) for localized topical treatment of SSI. The FTIR, XRD, DSC and TGA of bandage confirmed the compatibility of ingredients and modifications made. The porosity, swelling index and lysozyme degradation showed good properties for wound healing and biodegradation. Moreover, in-vitro antibacterial activity showed higher bactericidal effect as compared to ZnO-NPs free bandage. In-vivo wound healing in murine model showed significant improved tissue generation and speedy wound healing as compared to positive and negative controls. Over all, thiolated bandage showed potential as an advanced therapeutic agent for treating surgical site infections, meeting the required features of an ideal surgical dressing.
url https://doi.org/10.1371/journal.pone.0217079
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