Quantification of drug-induced fractional killing using high-throughput microscopy

Quantification of drug-induced fractional killing using high-throughput microscopy

Summary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging. This protocol can be used to compare the effect of hundreds of conditions in parallel. We...

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Bibliographic Details
Main Authors: Zintis Inde, Jason Rodencal, Scott J. Dixon
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell biology
Cell-based assays
Cancer
High-throughput screening
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000071
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spelling doaj-9b93a86502e14eb2bc30c30d6a2a23ea2021-03-22T12:53:06ZengElsevierSTAR Protocols2666-16672021-03-0121100300Quantification of drug-induced fractional killing using high-throughput microscopyZintis Inde0Jason Rodencal1Scott J. Dixon2Department of Biology, Stanford University, 327 Campus Dr, Stanford, CA 94305, USA; Corresponding authorDepartment of Biology, Stanford University, 327 Campus Dr, Stanford, CA 94305, USADepartment of Biology, Stanford University, 327 Campus Dr, Stanford, CA 94305, USA; Corresponding authorSummary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging. This protocol can be used to compare the effect of hundreds of conditions in parallel. We show how this protocol can be used to examine fractional killing in response to inhibitors of the mitogen-activated protein kinase pathway.For complete details on the use and execution of this protocol, please refer to Inde et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721000071Cell biologyCell-based assaysCancerHigh-throughput screeningMicroscopy
collection DOAJ
language English
format Article
sources DOAJ
author Zintis Inde
Jason Rodencal
Scott J. Dixon
spellingShingle Zintis Inde
Jason Rodencal
Scott J. Dixon
Quantification of drug-induced fractional killing using high-throughput microscopy
STAR Protocols
Cell biology
Cell-based assays
Cancer
High-throughput screening
Microscopy
author_facet Zintis Inde
Jason Rodencal
Scott J. Dixon
author_sort Zintis Inde
title Quantification of drug-induced fractional killing using high-throughput microscopy
title_short Quantification of drug-induced fractional killing using high-throughput microscopy
title_full Quantification of drug-induced fractional killing using high-throughput microscopy
title_fullStr Quantification of drug-induced fractional killing using high-throughput microscopy
title_full_unstemmed Quantification of drug-induced fractional killing using high-throughput microscopy
title_sort quantification of drug-induced fractional killing using high-throughput microscopy
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging. This protocol can be used to compare the effect of hundreds of conditions in parallel. We show how this protocol can be used to examine fractional killing in response to inhibitors of the mitogen-activated protein kinase pathway.For complete details on the use and execution of this protocol, please refer to Inde et al. (2020).
topic Cell biology
Cell-based assays
Cancer
High-throughput screening
Microscopy
url http://www.sciencedirect.com/science/article/pii/S2666166721000071
work_keys_str_mv AT zintisinde quantificationofdruginducedfractionalkillingusinghighthroughputmicroscopy
AT jasonrodencal quantificationofdruginducedfractionalkillingusinghighthroughputmicroscopy
AT scottjdixon quantificationofdruginducedfractionalkillingusinghighthroughputmicroscopy
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