Molecular marker for screening yellow mosaic disease resistance in blackgram [Vigna mungo (L.) Hepper]

• Main Author: L.Prasanthi, B.V.Bhaskara Reddy, B.Geetha, Ramya Jyothi and Abhishek
• Format: Article
• Language: English
• Published: Indian Society of Plant Breeders 2013-06-01
• Series: Electronic Journal of Plant Breeding
• Subjects: Blackgram; YMD disease screening; SCAR marker
• Online Access: https://sites.google.com/site/ejpb2011/vol-4-2/EJPB_V4_N2_1137_1141.pdf?attredirects=0&d=1

Yellow mosaic disease is a serious disease in blackgram which causes severe yield losses. Screening of 45 lines along with PU-31 and PU-19 resistant checks for YMD under field conditions with artificial inoculation, identified 19 lines having 1 score with no disease symptoms. PCR reactions using SCAR marker for screening the disease reaction with genomic DNA of these lines resulted in identification of 19 resistant sources with specific amplification for resistance to YMV at 532bp with SCAR 20F/20R developed from OPQ1 RARD primer linked to YMV disease. Considering the YMV reaction and resistance linked SCAR marker, it is possible to identify the new resistance sources in a short time and they can be utilized in breeding programme or for direct release.