Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors

Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors

Characterization of synthetic oligonucleotides and quantification of primer extension mediated by a human translesion synthesis polymerase η (Pol η) over drug-induced DNA lesions in the presence on modified nucleotide analogs is described. Extent of primer extension for each reaction was monitored b...

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Main Authors: S. Malvezzi, S.J. Sturla, M. Tanasova
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340915000487
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spelling doaj-9b2dc403b8734b3f9f26aaa25ff8a75e2020-11-24T20:42:53ZengElsevierData in Brief2352-34092015-09-014C141810.1016/j.dib.2015.04.006Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitorsS. Malvezzi0S.J. Sturla1M. Tanasova2Department of Health Sciences and Technology, ETH Zurich, SwitzerlandDepartment of Health Sciences and Technology, ETH Zurich, SwitzerlandDepartment of Chemistry, Michigan Technological University, United StatesCharacterization of synthetic oligonucleotides and quantification of primer extension mediated by a human translesion synthesis polymerase η (Pol η) over drug-induced DNA lesions in the presence on modified nucleotide analogs is described. Extent of primer extension for each reaction was monitored by denaturing gel electrophoresis. The data was obtained to assess the performance of the fluorescence-based primer extension (PE-PiPer) assay [1] with respect to the established and conventionally used denaturing gel electrophoresis. The obtained data reflects the specific inhibition of translesion synthesis over cisplatin containing DNA with 5-OH-CTP.http://www.sciencedirect.com/science/article/pii/S2352340915000487
collection DOAJ
language English
format Article
sources DOAJ
author S. Malvezzi
S.J. Sturla
M. Tanasova
spellingShingle S. Malvezzi
S.J. Sturla
M. Tanasova
Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
Data in Brief
author_facet S. Malvezzi
S.J. Sturla
M. Tanasova
author_sort S. Malvezzi
title Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
title_short Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
title_full Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
title_fullStr Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
title_full_unstemmed Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
title_sort data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2015-09-01
description Characterization of synthetic oligonucleotides and quantification of primer extension mediated by a human translesion synthesis polymerase η (Pol η) over drug-induced DNA lesions in the presence on modified nucleotide analogs is described. Extent of primer extension for each reaction was monitored by denaturing gel electrophoresis. The data was obtained to assess the performance of the fluorescence-based primer extension (PE-PiPer) assay [1] with respect to the established and conventionally used denaturing gel electrophoresis. The obtained data reflects the specific inhibition of translesion synthesis over cisplatin containing DNA with 5-OH-CTP.
url http://www.sciencedirect.com/science/article/pii/S2352340915000487
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AT sjsturla datainsupportofquantificationofpyrophosphateasauniversalapproachtodeterminepolymeraseactivityandassaypolymeraseinhibitors
AT mtanasova datainsupportofquantificationofpyrophosphateasauniversalapproachtodeterminepolymeraseactivityandassaypolymeraseinhibitors
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