Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical Tissues

Vascular endothelial growth factor (VEGF)-induced angiogenesis contributes to inflammatory bone resorption in humans. Widely documented antagonists to resorption include antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs). The purpose of this study was to investigate the effect of these d...

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Main Authors: Aleksandra Palatyńska-Ulatowska, Marta Michalska, Anna Drelich, Aleksandra Sałagacka-Kubiak, Ewa Balcerczak, Bogusława Manowska, José Antonio Poli de Figueiredo
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/11/4976
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spelling doaj-9b2d61e869274572baa6b5d8686af8322021-06-01T01:27:55ZengMDPI AGApplied Sciences2076-34172021-05-01114976497610.3390/app11114976Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical TissuesAleksandra Palatyńska-Ulatowska0Marta Michalska1Anna Drelich2Aleksandra Sałagacka-Kubiak3Ewa Balcerczak4Bogusława Manowska5José Antonio Poli de Figueiredo6Department of Endodontics, Chair of Conservative Dentistry and Endodontics, Medical University of Lodz, 251 Pomorska Str., 92-217 Lodz, PolandDepartment of Pharmaceutical Biochemistry, Medical University of Lodz, 1 Muszyńskiego Str., 90-151 Lodz, PolandDepartment of Pharmaceutical Biochemistry, Medical University of Lodz, 1 Muszyńskiego Str., 90-151 Lodz, PolandLaboratory of Molecular Diagnostics and Pharmacogenomics, Department of Pharmaceutical Biochemistry and Molecular Diagnostics, Medical University of Lodz, 1 Muszyńskiego Str., 90-151 Lodz, PolandLaboratory of Molecular Diagnostics and Pharmacogenomics, Department of Pharmaceutical Biochemistry and Molecular Diagnostics, Medical University of Lodz, 1 Muszyńskiego Str., 90-151 Lodz, PolandDepartment of Maxillofacial Surgery, Medical University of Lodz, 113th S. Żeromskiego, 90-549 Lodz, PolandDepartment of Morphological Sciences, Federal University of Rio Grande do Sul-UFRGS, Rua Sarmento Leite 500 sala 134, Porto Alegre CEP 90050-170, BrazilVascular endothelial growth factor (VEGF)-induced angiogenesis contributes to inflammatory bone resorption in humans. Widely documented antagonists to resorption include antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs). The purpose of this study was to investigate the effect of these drugs on proangiogenic VEGF levels in periradicular lesions. Periapical tissue biopsies were obtained from 42 patients with chronic periapical periodontitis. VEGF levels were measured using a commercial ELISA kit in patients divided into groups according to treatment: no drugs (control group, <i>n</i> = 25), NSAIDs (<i>n</i> = 7), antibiotics (<i>n</i> = 5), and NSAIDs and antibiotics (<i>n</i> = 5). Reverse transcriptase (RT) reaction was performed in all the samples under analysis. Presence of <i>VEGFA</i> and <i>VEGFB</i> gene expression was assessed using reverse-transcription-polymerase chain reaction (RT-PCR). ELISA analysis indicated that average VEGF levels in tissue samples of patients treated with NSAIDs (6.097 ± 1.930 ng/mL), antibiotics (5.661 ± 2.395 ng/mL), and NSAIDs and antibiotics (7.142 ± 2.601 ng/mL) were significantly lower than in samples of control patients (10.432 ± 4.257 ng/mL, ANOVA <i>p</i> = 0.008). The RT-PCR did not reveal <i>VEGFA</i> gene expression in any of the 42 samples. <i>VEGFB</i> gene expression was found in 26 of 42 samples (69.1%). The use of NSAIDs or antibiotics in patients with exacerbated chronic periodontitis decreases VEGF levels in periapical tissues. Pharmacotherapy may minimize the effects of VEGF on apical periodontitis progression in that way.https://www.mdpi.com/2076-3417/11/11/4976endodontic treatmentperiapical periodontitisantibioticsnonsteroidal anti-inflammatory drugspolymerase chain reaction (PCR)vascular endothelial growth factor
collection DOAJ
language English
format Article
sources DOAJ
author Aleksandra Palatyńska-Ulatowska
Marta Michalska
Anna Drelich
Aleksandra Sałagacka-Kubiak
Ewa Balcerczak
Bogusława Manowska
José Antonio Poli de Figueiredo
spellingShingle Aleksandra Palatyńska-Ulatowska
Marta Michalska
Anna Drelich
Aleksandra Sałagacka-Kubiak
Ewa Balcerczak
Bogusława Manowska
José Antonio Poli de Figueiredo
Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical Tissues
Applied Sciences
endodontic treatment
periapical periodontitis
antibiotics
nonsteroidal anti-inflammatory drugs
polymerase chain reaction (PCR)
vascular endothelial growth factor
author_facet Aleksandra Palatyńska-Ulatowska
Marta Michalska
Anna Drelich
Aleksandra Sałagacka-Kubiak
Ewa Balcerczak
Bogusława Manowska
José Antonio Poli de Figueiredo
author_sort Aleksandra Palatyńska-Ulatowska
title Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical Tissues
title_short Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical Tissues
title_full Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical Tissues
title_fullStr Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical Tissues
title_full_unstemmed Systemic Antibiotic and Nonsteroidal Anti-Inflammatory Drug Treatment Decreases the Level of Endogenous Angiogenic Vascular Endothelial Growth Factor in Inflamed Human Periapical Tissues
title_sort systemic antibiotic and nonsteroidal anti-inflammatory drug treatment decreases the level of endogenous angiogenic vascular endothelial growth factor in inflamed human periapical tissues
publisher MDPI AG
series Applied Sciences
issn 2076-3417
publishDate 2021-05-01
description Vascular endothelial growth factor (VEGF)-induced angiogenesis contributes to inflammatory bone resorption in humans. Widely documented antagonists to resorption include antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs). The purpose of this study was to investigate the effect of these drugs on proangiogenic VEGF levels in periradicular lesions. Periapical tissue biopsies were obtained from 42 patients with chronic periapical periodontitis. VEGF levels were measured using a commercial ELISA kit in patients divided into groups according to treatment: no drugs (control group, <i>n</i> = 25), NSAIDs (<i>n</i> = 7), antibiotics (<i>n</i> = 5), and NSAIDs and antibiotics (<i>n</i> = 5). Reverse transcriptase (RT) reaction was performed in all the samples under analysis. Presence of <i>VEGFA</i> and <i>VEGFB</i> gene expression was assessed using reverse-transcription-polymerase chain reaction (RT-PCR). ELISA analysis indicated that average VEGF levels in tissue samples of patients treated with NSAIDs (6.097 ± 1.930 ng/mL), antibiotics (5.661 ± 2.395 ng/mL), and NSAIDs and antibiotics (7.142 ± 2.601 ng/mL) were significantly lower than in samples of control patients (10.432 ± 4.257 ng/mL, ANOVA <i>p</i> = 0.008). The RT-PCR did not reveal <i>VEGFA</i> gene expression in any of the 42 samples. <i>VEGFB</i> gene expression was found in 26 of 42 samples (69.1%). The use of NSAIDs or antibiotics in patients with exacerbated chronic periodontitis decreases VEGF levels in periapical tissues. Pharmacotherapy may minimize the effects of VEGF on apical periodontitis progression in that way.
topic endodontic treatment
periapical periodontitis
antibiotics
nonsteroidal anti-inflammatory drugs
polymerase chain reaction (PCR)
vascular endothelial growth factor
url https://www.mdpi.com/2076-3417/11/11/4976
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