Copy Number Deletion Has Little Impact on Gene Expression Levels in Racehorses

• Main Authors: Kyung-Do Park, Hyeongmin Kim, Jae Yeon Hwang, Chang-Kyu Lee, Kyoung-Tag Do, Heui-Soo Kim, Young-Mok Yang, Young-jun Kwon, Jaemin Kim, Hyeon Jeong Kim, Ki-Duk Song, Jae-Don Oh, Heebal Kim, Byung-Wook Cho, Seoae Cho, Hak-Kyo Lee
• Format: Article
• Language: English
• Published: Asian-Australasian Association of Animal Production Societies 2014-09-01
• Series: Asian-Australasian Journal of Animal Sciences
• Subjects: Copy Number Variation; Genome-wide Expression; Horse; Thoroughbred
• Online Access: http://www.ajas.info/upload/pdf/ajas-27-9-1345.pdf
• ISSN: 1011-2367; 1976-5517

Copy number variations (CNVs), important genetic factors for study of human diseases, may have as large of an effect on phenotype as do single nucleotide polymorphisms. Indeed, it is widely accepted that CNVs are associated with differential disease susceptibility. However, the relationships between CNVs and gene expression have not been characterized in the horse. In this study, we investigated the effects of copy number deletion in the blood and muscle transcriptomes of Thoroughbred racing horses. We identified a total of 1,246 CNVs of deletion polymorphisms using DNA re-sequencing data from 18 Thoroughbred racing horses. To discover the tendencies between CNV status and gene expression levels, we extracted CNVs of four Thoroughbred racing horses of which RNA sequencing was available. We found that 252 pairs of CNVs and genes were associated in the four horse samples. We did not observe a clear and consistent relationship between the deletion status of CNVs and gene expression levels before and after exercise in blood and muscle. However, we found some pairs of CNVs and associated genes that indicated relationships with gene expression levels: a positive relationship with genes responsible for membrane structure or cytoskeleton and a negative relationship with genes involved in disease. This study will lead to conceptual advances in understanding the relationship between CNVs and global gene expression in the horse.