Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation

Pomegranates are widely consumed either as fresh fruit or in beverage form as juice and wine. Ellagic acid possesses potent antioxidative properties; it is known to be an effective phytotherapeutic agent with antimutagenic and anticarcinogenic qualities. Ellagic acid (20 to 80 μM) exhibited a potent...

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Main Authors: Yi Chang, Wei-Fan Chen, Kuan-Hung Lin, Cheng-Ying Hsieh, Duen-Suey Chou, Li-Jyun Lin, Joen-Rong Sheu, Chao-Chien Chang
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2013/595128
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spelling doaj-9b06f181457d43d6b5ba200a592d81e82020-11-24T21:31:52ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882013-01-01201310.1155/2013/595128595128Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet ActivationYi Chang0Wei-Fan Chen1Kuan-Hung Lin2Cheng-Ying Hsieh3Duen-Suey Chou4Li-Jyun Lin5Joen-Rong Sheu6Chao-Chien Chang7Department of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanDepartment of Pharmacology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanDepartment of Pharmacology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanDepartment of Pharmacology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanPomegranates are widely consumed either as fresh fruit or in beverage form as juice and wine. Ellagic acid possesses potent antioxidative properties; it is known to be an effective phytotherapeutic agent with antimutagenic and anticarcinogenic qualities. Ellagic acid (20 to 80 μM) exhibited a potent activity in inhibiting platelet aggregation stimulated by collagen; however, it did not inhibit platelet aggregation stimulated by thrombin, arachidonic acid, or U46619. Treatment with ellagic acid (50 and 80 μM) significantly inhibited platelet activation stimulated by collagen; this alteration was accompanied by the inhibition of relative [Ca2+]i mobilization, and the phosphorylation of phospholipase C (PLC)γ2, protein kinase C (PKC), mitogen-activated protein kinases (MAPKs), and Akt, as well as hydroxyl radical (OH●) formation. In addition, ellagic acid also inhibited p38 MAPK and Akt phosphorylation stimulated by hydrogen peroxide. By contrast, ellagic acid did not significantly affect PKC activation and platelet aggregation stimulated by PDBu. This study is the first to show that, in addition to being considered a possible agent for preventing tumor growth, ellagic acid possesses potent antiplatelet properties. It appears to initially inhibit the PLCγ2-PKC cascade and/or hydroxyl radical formation, followed by decreased phosphorylation of MAPKs and Akt, ultimately inhibiting platelet aggregation.http://dx.doi.org/10.1155/2013/595128
collection DOAJ
language English
format Article
sources DOAJ
author Yi Chang
Wei-Fan Chen
Kuan-Hung Lin
Cheng-Ying Hsieh
Duen-Suey Chou
Li-Jyun Lin
Joen-Rong Sheu
Chao-Chien Chang
spellingShingle Yi Chang
Wei-Fan Chen
Kuan-Hung Lin
Cheng-Ying Hsieh
Duen-Suey Chou
Li-Jyun Lin
Joen-Rong Sheu
Chao-Chien Chang
Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
Evidence-Based Complementary and Alternative Medicine
author_facet Yi Chang
Wei-Fan Chen
Kuan-Hung Lin
Cheng-Ying Hsieh
Duen-Suey Chou
Li-Jyun Lin
Joen-Rong Sheu
Chao-Chien Chang
author_sort Yi Chang
title Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
title_short Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
title_full Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
title_fullStr Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
title_full_unstemmed Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
title_sort novel bioactivity of ellagic acid in inhibiting human platelet activation
publisher Hindawi Limited
series Evidence-Based Complementary and Alternative Medicine
issn 1741-427X
1741-4288
publishDate 2013-01-01
description Pomegranates are widely consumed either as fresh fruit or in beverage form as juice and wine. Ellagic acid possesses potent antioxidative properties; it is known to be an effective phytotherapeutic agent with antimutagenic and anticarcinogenic qualities. Ellagic acid (20 to 80 μM) exhibited a potent activity in inhibiting platelet aggregation stimulated by collagen; however, it did not inhibit platelet aggregation stimulated by thrombin, arachidonic acid, or U46619. Treatment with ellagic acid (50 and 80 μM) significantly inhibited platelet activation stimulated by collagen; this alteration was accompanied by the inhibition of relative [Ca2+]i mobilization, and the phosphorylation of phospholipase C (PLC)γ2, protein kinase C (PKC), mitogen-activated protein kinases (MAPKs), and Akt, as well as hydroxyl radical (OH●) formation. In addition, ellagic acid also inhibited p38 MAPK and Akt phosphorylation stimulated by hydrogen peroxide. By contrast, ellagic acid did not significantly affect PKC activation and platelet aggregation stimulated by PDBu. This study is the first to show that, in addition to being considered a possible agent for preventing tumor growth, ellagic acid possesses potent antiplatelet properties. It appears to initially inhibit the PLCγ2-PKC cascade and/or hydroxyl radical formation, followed by decreased phosphorylation of MAPKs and Akt, ultimately inhibiting platelet aggregation.
url http://dx.doi.org/10.1155/2013/595128
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