Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
Pomegranates are widely consumed either as fresh fruit or in beverage form as juice and wine. Ellagic acid possesses potent antioxidative properties; it is known to be an effective phytotherapeutic agent with antimutagenic and anticarcinogenic qualities. Ellagic acid (20 to 80 μM) exhibited a potent...
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doaj-9b06f181457d43d6b5ba200a592d81e82020-11-24T21:31:52ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882013-01-01201310.1155/2013/595128595128Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet ActivationYi Chang0Wei-Fan Chen1Kuan-Hung Lin2Cheng-Ying Hsieh3Duen-Suey Chou4Li-Jyun Lin5Joen-Rong Sheu6Chao-Chien Chang7Department of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanDepartment of Pharmacology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanDepartment of Pharmacology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanGraduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanDepartment of Pharmacology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, TaiwanPomegranates are widely consumed either as fresh fruit or in beverage form as juice and wine. Ellagic acid possesses potent antioxidative properties; it is known to be an effective phytotherapeutic agent with antimutagenic and anticarcinogenic qualities. Ellagic acid (20 to 80 μM) exhibited a potent activity in inhibiting platelet aggregation stimulated by collagen; however, it did not inhibit platelet aggregation stimulated by thrombin, arachidonic acid, or U46619. Treatment with ellagic acid (50 and 80 μM) significantly inhibited platelet activation stimulated by collagen; this alteration was accompanied by the inhibition of relative [Ca2+]i mobilization, and the phosphorylation of phospholipase C (PLC)γ2, protein kinase C (PKC), mitogen-activated protein kinases (MAPKs), and Akt, as well as hydroxyl radical (OH●) formation. In addition, ellagic acid also inhibited p38 MAPK and Akt phosphorylation stimulated by hydrogen peroxide. By contrast, ellagic acid did not significantly affect PKC activation and platelet aggregation stimulated by PDBu. This study is the first to show that, in addition to being considered a possible agent for preventing tumor growth, ellagic acid possesses potent antiplatelet properties. It appears to initially inhibit the PLCγ2-PKC cascade and/or hydroxyl radical formation, followed by decreased phosphorylation of MAPKs and Akt, ultimately inhibiting platelet aggregation.http://dx.doi.org/10.1155/2013/595128 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi Chang Wei-Fan Chen Kuan-Hung Lin Cheng-Ying Hsieh Duen-Suey Chou Li-Jyun Lin Joen-Rong Sheu Chao-Chien Chang |
spellingShingle |
Yi Chang Wei-Fan Chen Kuan-Hung Lin Cheng-Ying Hsieh Duen-Suey Chou Li-Jyun Lin Joen-Rong Sheu Chao-Chien Chang Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation Evidence-Based Complementary and Alternative Medicine |
author_facet |
Yi Chang Wei-Fan Chen Kuan-Hung Lin Cheng-Ying Hsieh Duen-Suey Chou Li-Jyun Lin Joen-Rong Sheu Chao-Chien Chang |
author_sort |
Yi Chang |
title |
Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation |
title_short |
Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation |
title_full |
Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation |
title_fullStr |
Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation |
title_full_unstemmed |
Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation |
title_sort |
novel bioactivity of ellagic acid in inhibiting human platelet activation |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2013-01-01 |
description |
Pomegranates are widely consumed either as fresh fruit or in beverage form as juice and wine. Ellagic acid possesses potent antioxidative properties; it is known to be an effective phytotherapeutic agent with antimutagenic and anticarcinogenic qualities. Ellagic acid (20 to 80 μM) exhibited a potent activity in inhibiting platelet aggregation stimulated by collagen; however, it did not inhibit platelet aggregation stimulated by thrombin, arachidonic acid, or U46619. Treatment with ellagic acid (50 and 80 μM) significantly inhibited platelet activation stimulated by collagen; this alteration was accompanied by the inhibition of relative [Ca2+]i mobilization, and the phosphorylation of phospholipase C (PLC)γ2, protein kinase C (PKC), mitogen-activated protein kinases (MAPKs), and Akt, as well as hydroxyl radical (OH●) formation. In addition, ellagic acid also inhibited p38 MAPK and Akt phosphorylation stimulated by hydrogen peroxide. By contrast, ellagic acid did not significantly affect PKC activation and platelet aggregation stimulated by PDBu. This study is the first to show that, in addition to being considered a possible agent for preventing tumor growth, ellagic acid possesses potent antiplatelet properties. It appears to initially inhibit the PLCγ2-PKC cascade and/or hydroxyl radical formation, followed by decreased phosphorylation of MAPKs and Akt, ultimately inhibiting platelet aggregation. |
url |
http://dx.doi.org/10.1155/2013/595128 |
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