Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt
This study has established a successful protocol to cryopreserve lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt. Three cryosolutions were tested based on Mounib’s medium; the original medium including reduced l-glutathione (GSH), the basic sucrose and potassium bicarbonate medium without GSH, or...
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doaj-9b066eb68f7943f4a6587231b860a8bd2020-11-24T21:25:05ZengPeerJ Inc.PeerJ2167-83592015-06-013e100310.7717/peerj.10031003Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) miltGunnvør Norðberg0Asa Johannesen1Regin Arge2Fiskaaling, Aquacultural Research Station of the Faroes, við Áir, Hvalvík, Faroe IslandsFiskaaling, Aquacultural Research Station of the Faroes, við Áir, Hvalvík, Faroe IslandsFiskaaling, Aquacultural Research Station of the Faroes, við Áir, Hvalvík, Faroe IslandsThis study has established a successful protocol to cryopreserve lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt. Three cryosolutions were tested based on Mounib’s medium; the original medium including reduced l-glutathione (GSH), the basic sucrose and potassium bicarbonate medium without GSH, or with hen’s egg yolk (EY). Dimethyl sulphoxide (DMSO) was used as the cryoprotectant along with all three diluents in a 1–2 dilution. Cryopreservation was performed with the mentioned cryosolutions at two freezing rates. Motility percentages of spermatozoa were evaluated using ImageJ with a computer assisted sperm analyzer (CASA) plug-in. Findings revealed that spermatozoa cryopreserved in Mounib’s medium without GSH had a post-thaw motility score of 6.4 percentage points (pp) higher than those in the original Mounib’s medium, and an addition of EY to the modified Mounib’s medium lowered the post-thaw motility score by 19.3 pp. The difference in motility between both freezing rates was 13.0 pp, and samples cryopreserved on a 4.8 cm high tray resulted in a better post-thaw motility score. On average, cryopreserved milt had a 24.1 pp lower post-thaw motility score than fresh milt. There was no significant difference in fertilisation success between cryopreserved and fresh milt. Cryopreservation of lumpfish milt has, to our knowledge, never been successfully carried out before. The established protocol will be a main contributing factor in a stable production of lumpfish juveniles in future.https://peerj.com/articles/1003.pdfLumpfish Cyclopterus lumpus CryopreservationMilt motilityCASA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gunnvør Norðberg Asa Johannesen Regin Arge |
spellingShingle |
Gunnvør Norðberg Asa Johannesen Regin Arge Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt PeerJ Lumpfish Cyclopterus lumpus Cryopreservation Milt motility CASA |
author_facet |
Gunnvør Norðberg Asa Johannesen Regin Arge |
author_sort |
Gunnvør Norðberg |
title |
Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt |
title_short |
Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt |
title_full |
Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt |
title_fullStr |
Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt |
title_full_unstemmed |
Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt |
title_sort |
cryopreservation of lumpfish cyclopterus lumpus (linnaeus, 1758) milt |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2015-06-01 |
description |
This study has established a successful protocol to cryopreserve lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt. Three cryosolutions were tested based on Mounib’s medium; the original medium including reduced l-glutathione (GSH), the basic sucrose and potassium bicarbonate medium without GSH, or with hen’s egg yolk (EY). Dimethyl sulphoxide (DMSO) was used as the cryoprotectant along with all three diluents in a 1–2 dilution. Cryopreservation was performed with the mentioned cryosolutions at two freezing rates. Motility percentages of spermatozoa were evaluated using ImageJ with a computer assisted sperm analyzer (CASA) plug-in. Findings revealed that spermatozoa cryopreserved in Mounib’s medium without GSH had a post-thaw motility score of 6.4 percentage points (pp) higher than those in the original Mounib’s medium, and an addition of EY to the modified Mounib’s medium lowered the post-thaw motility score by 19.3 pp. The difference in motility between both freezing rates was 13.0 pp, and samples cryopreserved on a 4.8 cm high tray resulted in a better post-thaw motility score. On average, cryopreserved milt had a 24.1 pp lower post-thaw motility score than fresh milt. There was no significant difference in fertilisation success between cryopreserved and fresh milt. Cryopreservation of lumpfish milt has, to our knowledge, never been successfully carried out before. The established protocol will be a main contributing factor in a stable production of lumpfish juveniles in future. |
topic |
Lumpfish Cyclopterus lumpus Cryopreservation Milt motility CASA |
url |
https://peerj.com/articles/1003.pdf |
work_keys_str_mv |
AT gunnvørnorðberg cryopreservationoflumpfishcyclopteruslumpuslinnaeus1758milt AT asajohannesen cryopreservationoflumpfishcyclopteruslumpuslinnaeus1758milt AT reginarge cryopreservationoflumpfishcyclopteruslumpuslinnaeus1758milt |
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