Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt

This study has established a successful protocol to cryopreserve lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt. Three cryosolutions were tested based on Mounib’s medium; the original medium including reduced l-glutathione (GSH), the basic sucrose and potassium bicarbonate medium without GSH, or...

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Main Authors: Gunnvør Norðberg, Asa Johannesen, Regin Arge
Format: Article
Language:English
Published: PeerJ Inc. 2015-06-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/1003.pdf
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spelling doaj-9b066eb68f7943f4a6587231b860a8bd2020-11-24T21:25:05ZengPeerJ Inc.PeerJ2167-83592015-06-013e100310.7717/peerj.10031003Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) miltGunnvør Norðberg0Asa Johannesen1Regin Arge2Fiskaaling, Aquacultural Research Station of the Faroes, við Áir, Hvalvík, Faroe IslandsFiskaaling, Aquacultural Research Station of the Faroes, við Áir, Hvalvík, Faroe IslandsFiskaaling, Aquacultural Research Station of the Faroes, við Áir, Hvalvík, Faroe IslandsThis study has established a successful protocol to cryopreserve lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt. Three cryosolutions were tested based on Mounib’s medium; the original medium including reduced l-glutathione (GSH), the basic sucrose and potassium bicarbonate medium without GSH, or with hen’s egg yolk (EY). Dimethyl sulphoxide (DMSO) was used as the cryoprotectant along with all three diluents in a 1–2 dilution. Cryopreservation was performed with the mentioned cryosolutions at two freezing rates. Motility percentages of spermatozoa were evaluated using ImageJ with a computer assisted sperm analyzer (CASA) plug-in. Findings revealed that spermatozoa cryopreserved in Mounib’s medium without GSH had a post-thaw motility score of 6.4 percentage points (pp) higher than those in the original Mounib’s medium, and an addition of EY to the modified Mounib’s medium lowered the post-thaw motility score by 19.3 pp. The difference in motility between both freezing rates was 13.0 pp, and samples cryopreserved on a 4.8 cm high tray resulted in a better post-thaw motility score. On average, cryopreserved milt had a 24.1 pp lower post-thaw motility score than fresh milt. There was no significant difference in fertilisation success between cryopreserved and fresh milt. Cryopreservation of lumpfish milt has, to our knowledge, never been successfully carried out before. The established protocol will be a main contributing factor in a stable production of lumpfish juveniles in future.https://peerj.com/articles/1003.pdfLumpfish Cyclopterus lumpus CryopreservationMilt motilityCASA
collection DOAJ
language English
format Article
sources DOAJ
author Gunnvør Norðberg
Asa Johannesen
Regin Arge
spellingShingle Gunnvør Norðberg
Asa Johannesen
Regin Arge
Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt
PeerJ
Lumpfish
Cyclopterus lumpus
Cryopreservation
Milt motility
CASA
author_facet Gunnvør Norðberg
Asa Johannesen
Regin Arge
author_sort Gunnvør Norðberg
title Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt
title_short Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt
title_full Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt
title_fullStr Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt
title_full_unstemmed Cryopreservation of lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt
title_sort cryopreservation of lumpfish cyclopterus lumpus (linnaeus, 1758) milt
publisher PeerJ Inc.
series PeerJ
issn 2167-8359
publishDate 2015-06-01
description This study has established a successful protocol to cryopreserve lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt. Three cryosolutions were tested based on Mounib’s medium; the original medium including reduced l-glutathione (GSH), the basic sucrose and potassium bicarbonate medium without GSH, or with hen’s egg yolk (EY). Dimethyl sulphoxide (DMSO) was used as the cryoprotectant along with all three diluents in a 1–2 dilution. Cryopreservation was performed with the mentioned cryosolutions at two freezing rates. Motility percentages of spermatozoa were evaluated using ImageJ with a computer assisted sperm analyzer (CASA) plug-in. Findings revealed that spermatozoa cryopreserved in Mounib’s medium without GSH had a post-thaw motility score of 6.4 percentage points (pp) higher than those in the original Mounib’s medium, and an addition of EY to the modified Mounib’s medium lowered the post-thaw motility score by 19.3 pp. The difference in motility between both freezing rates was 13.0 pp, and samples cryopreserved on a 4.8 cm high tray resulted in a better post-thaw motility score. On average, cryopreserved milt had a 24.1 pp lower post-thaw motility score than fresh milt. There was no significant difference in fertilisation success between cryopreserved and fresh milt. Cryopreservation of lumpfish milt has, to our knowledge, never been successfully carried out before. The established protocol will be a main contributing factor in a stable production of lumpfish juveniles in future.
topic Lumpfish
Cyclopterus lumpus
Cryopreservation
Milt motility
CASA
url https://peerj.com/articles/1003.pdf
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