Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells
Pneumonia is a serious respiratory tract infection disease in children, which threatens to the health or life of children patients. Ginsenoside Rb1 (Rb1) is a principle active ingredient extracted from the root of Panax notoginseng (Burk.) F.H. Chen with anti-inflammatory effect. Our study aimed to...
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Online Access: | https://doi.org/10.1177/09636897211002787 |
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doaj-9afc16de918f4071ac12bf9b35f1e55b2021-04-28T22:03:28ZengSAGE PublishingCell Transplantation1555-38922021-04-013010.1177/09636897211002787Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 CellsErhu Wei0Xiao Fang1Peisheng Jia2Mingxia Li3Peina Jin4Fengyan Li5Huaili Wang6Dan Gao7 Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Department of Nephrology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaPneumonia is a serious respiratory tract infection disease in children, which threatens to the health or life of children patients. Ginsenoside Rb1 (Rb1) is a principle active ingredient extracted from the root of Panax notoginseng (Burk.) F.H. Chen with anti-inflammatory effect. Our study aimed to determine the effects and molecular mechanisms of Rb1 on lipopolysaccharide (LPS)-induced inflammatory injury of lung fibroblasts WI-38 cells. Cell viability and apoptosis were evaluated by CCK-8 and flow cytometry, respectively. The production of inflammatory cytokines were measured by ELISA and RT-qPCR. miR-222 expression was examined by RT-qPCR. The expression levels of the nuclear factor-kappa B (NF-κB) p65 and phosphorylated p65 were detected by western blot. We found that LPS stimulation induced WI-38 cell inflammatory injury by inhibiting cell viability, and inducing apoptosis and inflammatory cytokine production, while treatment with Rb1 significantly attenuated LPS-induced inflammatory injury in WI-38 cells. Additionally, Rb1 decreased LPS-induced upregulation of miR-222 and activation of the NF-κB pathway in WI-38 cells. Overexpression of miR-222 abolished the inhibitory effects of Rb1 on LPS-induced viability reduction, apoptosis, inflammatory cytokine production and activation of the NF-κB pathway. In conclusion, Rb1 alleviated LPS-induced inflammatory injury in WI-38 cells via downregulating miR-222 and inactivation of the NF-kB pathway.https://doi.org/10.1177/09636897211002787 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Erhu Wei Xiao Fang Peisheng Jia Mingxia Li Peina Jin Fengyan Li Huaili Wang Dan Gao |
spellingShingle |
Erhu Wei Xiao Fang Peisheng Jia Mingxia Li Peina Jin Fengyan Li Huaili Wang Dan Gao Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells Cell Transplantation |
author_facet |
Erhu Wei Xiao Fang Peisheng Jia Mingxia Li Peina Jin Fengyan Li Huaili Wang Dan Gao |
author_sort |
Erhu Wei |
title |
Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells |
title_short |
Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells |
title_full |
Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells |
title_fullStr |
Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells |
title_full_unstemmed |
Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells |
title_sort |
ginsenoside rb1 alleviates lipopolysaccharide-induced inflammatory injury by downregulating mir-222 in wi-38 cells |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
1555-3892 |
publishDate |
2021-04-01 |
description |
Pneumonia is a serious respiratory tract infection disease in children, which threatens to the health or life of children patients. Ginsenoside Rb1 (Rb1) is a principle active ingredient extracted from the root of Panax notoginseng (Burk.) F.H. Chen with anti-inflammatory effect. Our study aimed to determine the effects and molecular mechanisms of Rb1 on lipopolysaccharide (LPS)-induced inflammatory injury of lung fibroblasts WI-38 cells. Cell viability and apoptosis were evaluated by CCK-8 and flow cytometry, respectively. The production of inflammatory cytokines were measured by ELISA and RT-qPCR. miR-222 expression was examined by RT-qPCR. The expression levels of the nuclear factor-kappa B (NF-κB) p65 and phosphorylated p65 were detected by western blot. We found that LPS stimulation induced WI-38 cell inflammatory injury by inhibiting cell viability, and inducing apoptosis and inflammatory cytokine production, while treatment with Rb1 significantly attenuated LPS-induced inflammatory injury in WI-38 cells. Additionally, Rb1 decreased LPS-induced upregulation of miR-222 and activation of the NF-κB pathway in WI-38 cells. Overexpression of miR-222 abolished the inhibitory effects of Rb1 on LPS-induced viability reduction, apoptosis, inflammatory cytokine production and activation of the NF-κB pathway. In conclusion, Rb1 alleviated LPS-induced inflammatory injury in WI-38 cells via downregulating miR-222 and inactivation of the NF-kB pathway. |
url |
https://doi.org/10.1177/09636897211002787 |
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