Phylogenetic groups and cephalosporin resistance genes of <it>Escherichia coli </it>from diseased food-producing animals in Japan

• Main Authors: Ozawa Manao, Baba Kotaro, Usui Masaru, Hiki Mototaka, Sato Chizuru, Masani Kaori, Asai Tetsuo, Harada Kazuki, Aoki Hiroshi, Sawada Takuo
• Format: Article
• Language: English
• Published: BMC 2011-10-01
• Series: Acta Veterinaria Scandinavica
• Subjects: <it>Escherichia coli</it>; food-producing animals; cephalosporin resistance; extended spectrum β-lactamase; AmpC β-lactamase; phylogenetic grouping
• Online Access: http://www.actavetscand.com/content/53/1/52

<p>Abstract</p> <p>A total of 318 <it>Escherichia coli </it>isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of <it>E. coli </it>from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced <it>bla_CTX-M-2</it>, while the nine poultry isolates produced <it>bla_CTX-M-25</it>(4), <it>bla_SHV-2</it>(3), <it>bla_CTX-M-15</it>(1) and <it>bla_CTX-M-2</it>(1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in <it>E. coli </it>from diseased animals in Japan.</p>