Analysis of pollen-specific alternative splicing in Arabidopsis thaliana via semi-quantitative PCR

Alternative splicing enables a single gene to produce multiple mRNA isoforms by varying splice site selection. In animals, alternative splicing of mRNA isoforms between cell types is widespread and supports cellular differentiation. In plants, at least 20% of multi-exon genes are alternatively splic...

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Bibliographic Details
Main Authors: April D. Estrada, Nowlan H. Freese, Ivory C. Blakley, Ann E. Loraine
Format: Article
Language:English
Published: PeerJ Inc. 2015-04-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/919.pdf