Comparison of the antifungal effects of various extracts of Onosma Chlorotricum on Candida albicans and Candida glaberata with two antibiotics fluconazole and nystatin

• Main Authors: Fatemeh Nabipour, Behrouz Dousti
• Format: Article
• Language: fas
• Published: Lorestan University of Medical Science 2018-04-01
• Series: Yafteh
• Subjects: Fluconazole; nystatin; Antifungal; Extract; Onosma Chlorotricum
• Online Access: http://yafte.lums.ac.ir/browse.php?a_code=A-10-2037-1&slc_lang=en&sid=1

Background : Because of the recurrence of Candida infections, their resistance to antifungal drugs during treatment, the known side effects of chemical drugs, and the existence of some studies that Onosma's genus contains derivatives of alkannin and shikonin that have antifungal properties, for the first time, the antifungal properties of Onosma Chlorotricum were compared with antibiotics. Materials and Methods: The plant was collected from Koohdasht in July of 2016, and after detection and drying, N- hexane, methanol, aqueous extracts of plants were prepared by soaking. The antifungal effects of different concentrations of extracts were studied by the disc diffusion method and determination of MIC (minimum inhibitory concentration of growth), MFC (minimum fungicidal concentration) was done by microdilution method. Antibiotics fluconazole and nystatine were used as a positive control and DMSO was used as a negative control . Data were analyzed by t-test and one-way ANOVA. Results: The mean inhibitory diameter of growth of the hexane extract in concentration of 10 mg / ml was higher than nystatin antibiotic inhibitory diameter of growth for Candida albicans and Candida glaberata (P˂0.05). The lowest MIC for Candida albicans and Candida glaberata was respectively, 15.62 and 31.25 μg / μl, and the lowest MFC for Candida albicans and Candida glaberata was 31.25 and 62.5. μg / μl. Conclusion: Extracts of this plant showed antifungal effect on Candida albicans and glaberata. The possibility of using this extract in the treatment of Candida infection requires the completion of supplementary studies.