IGF-I activates caspases 3/7, 8 and 9 but does not induce cell death in colorectal cancer cells

• Main Authors: Seifalian Alexander M, Fuller Barry, Sales Kevin M, Bolvin Capucine, Yang Shi Yu, Winslet Marc C
• Format: Article
• Language: English
• Published: BMC 2009-05-01
• Series: BMC Cancer
• Online Access: http://www.biomedcentral.com/1471-2407/9/158

<p>Abstract</p> <p>Background</p> <p>Colorectal cancer is the third most common cancer in the western world. Chemotherapy is often ineffective to treat the advanced colorectal cancers due to the chemo-resistance. A major contributor to chemo-resistance is tumour-derived inhibition or avoidance of apoptosis. Insulin-like growth factor I (IGF-I) has been known to play a prominent role in colorectal cancer development and progression. The role of IGF-I in cancer cell apoptosis is not completely understood.</p> <p>Methods</p> <p>Using three colorectal cancer cell lines and one muscle cell line, associations between IGF-I and activities of caspase 3/7, 8 and 9 have been examined; the role of insulin-like growth factor I receptor (IGF-IR) in the caspase activation has been investigated.</p> <p>Results</p> <p>The results show that exogenous IGF-I significantly increases activity of caspases 3/7, 8 and 9 in all cell lines used; blocking IGF-I receptor reduce IGF-I-induced caspase activation. Further studies demonstrate that IGF-I induced caspase activation does not result in cell death. This is the first report to show that while IGF-I activates caspases 3/7, 8 and 9 it does not cause colorectal cancer cell death.</p> <p>Conclusion</p> <p>The study suggests that caspase activation is not synonymous with apoptosis and that activation of caspases may not necessarily induce cell death.</p>