The suppressive role of miR-362-3p in epithelial ovarian cancer

• Main Authors: Jialing Yuan, Tao Li, Ke Yi, Minmin Hou
• Format: Article
• Language: English
• Published: Elsevier 2020-07-01
• Series: Heliyon
• Subjects: Cell biology; Bioinformatics; Cancer research; Oncology; Clinical research; Ovarian cancer
• Online Access: http://www.sciencedirect.com/science/article/pii/S2405844020311026

Ovarian cancer is a common cancer worldwide. Epithelial ovarian cancer (EOC) is the most common subtype of ovarian cancer. This study was designed to explore the function of miR-362-3p in EOC. QRT-PCR analysis was used to test miR-362-3p levels in EOC tissues and cell lines. Cell viability was tested via MTT assay. Transwell systems were applied to assay cell migration. The target gene of miR-362-3p was evaluated using dual luciferase reporter assays. The MyD88 protein in EOC cells was tested via western blot. Our data showed that miR-362-3p was expressed at low levels in EOC tissues and cells. miR-362-3p inhibited cell proliferation and migration, bound the 3′-untranslated region (UTR) of MyD88, and inhibited MyD88 expression. MyD88 was inversely correlated with miR-362-3p in EOC, and MyD88 overexpression partly reduced the anti-proliferative effect of miR-362-3p in EOC cells. In conclusion, our data showed that miR-362-3p has an anti-proliferative effect on EOC.